RGD Reference Report - Sleep/Wake Physiology and Quantitative Electroencephalogram Analysis of the Neuroligin-3 Knockout Rat Model of Autism Spectrum Disorder. - Rat Genome Database

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Sleep/Wake Physiology and Quantitative Electroencephalogram Analysis of the Neuroligin-3 Knockout Rat Model of Autism Spectrum Disorder.

Authors: Thomas, Alexia M  Schwartz, Michael D  Saxe, Michael D  Kilduff, Thomas S 
Citation: Thomas AM, etal., Sleep. 2017 Oct 1;40(10). pii: 4100612. doi: 10.1093/sleep/zsx138.
RGD ID: 126790492
Pubmed: PMID:28958035   (View Abstract at PubMed)
DOI: DOI:10.1093/sleep/zsx138   (Journal Full-text)


Study Objectives: Neuroligin-3 (NLGN3) is one of the many genes associated with autism spectrum disorder (ASD). Sleep dysfunction is highly prevalent in ASD, but has not been rigorously examined in ASD models. Here, we evaluated sleep/wake physiology and behavioral phenotypes of rats with genetic ablation of Nlgn3.
Methods: Male Nlgn3 knockout (KO) and wild-type (WT) rats were assessed using a test battery for ASD-related behaviors and also implanted with telemeters to record the electroencephalogram (EEG), electromyogram, body temperature, and locomotor activity. 24-h EEG recordings were analyzed for sleep/wake states and spectral composition.
Results: Nlgn3 KO rats were hyperactive, exhibited excessive chewing behavior, and had impaired prepulse inhibition to an auditory startle stimulus. KO rats also spent less time in non-rapid eye movement (NREM) sleep, more time in rapid eye movement (REM) sleep, exhibited elevated theta power (4-9 Hz) during wakefulness and REM, and elevated delta power (0.5-4 Hz) during NREM. Beta (12-30 Hz) power and gamma (30-50 Hz) power were suppressed across all vigilance states.
Conclusions: The sleep disruptions in Nlgn3 KO rats are consistent with observations of sleep disturbances in ASD patients. The EEG provides objective measures of brain function to complement rodent behavioral analyses and therefore may be a useful tool to study ASD.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
autism spectrum disorder  ISONlgn3 (Rattus norvegicus)126790492; 126790492 RGD 
autism spectrum disorder  IMP 126790492; 126790492; 126790492 RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
circadian sleep/wake cycle  IMP 126790492 RGD 
prepulse inhibition  IMP 126790492 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nlgn3  (neuroligin 3)
Nlgn3em1Sage  (neuroligin 3; zinc finger nuclease induced mutant 1, Sigma Advanced Genetic Engineering Labs)

Genes (Mus musculus)
Nlgn3  (neuroligin 3)

Genes (Homo sapiens)
NLGN3  (neuroligin 3)

Strains
SD-Nlgn3em1Sage  (NA)


Additional Information