RGD Reference Report - Differential expression of RUNX genes in human esophageal squamous cell carcinoma: downregulation of RUNX3 worsens patient prognosis. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Differential expression of RUNX genes in human esophageal squamous cell carcinoma: downregulation of RUNX3 worsens patient prognosis.

Authors: Tonomoto, Yasuhito  Tachibana, Mitsuo  Dhar, Dipok Kumar  Onoda, Toshinao  Hata, Kohkichi  Ohnuma, Hideyuki  Tanaka, Tsuneo  Nagasue, Naofumi 
Citation: Tonomoto Y, etal., Oncology. 2007;73(5-6):346-56. doi: 10.1159/000135350. Epub 2008 May 26.
RGD ID: 126779568
Pubmed: (View Article at PubMed) PMID:18500170
DOI: Full-text: DOI:10.1159/000135350


BACKGROUND: The RUNX proteins are a family of transcriptional factors that have essential functions during embryogenesis and development, whereas deregulation in expression of RUNXs is often linked to tumor formation. To date, there has been no study describing the precise expression, prognostic impact and methylation status of RUNXs in esophageal squamous cell carcinoma.
METHODS: Resected specimens from 61 patients with esophageal SCC were used to identify the expression of RUNX1, RUNX2 and RUNX3 by real-time RT-PCR. Localization of mRNA was done by in situ hybridization, expression of Smad4 was evaluated by immunohistochemistry, and the methylation status of RUNX3 was analyzed by methylation-specific PCR.
RESULTS: RUNX3 had a significant impact on patient prognosis with worse survival in the RUNX3-negative group. In early tumors (T1/T2), the prevalence of lymph vessel invasion and the number of metastatic lymph nodes were significantly higher in RUNX3-negative tumors. Furthermore, RUNX3 became a strong prognostic factor only in Smad4-positive tumors. Also, the methylation status of the RUNX3 promoter had a significant correlation with the loss of RUNX3 expression.
CONCLUSION: Downregulation of RUNX3 may play a role in disease progression of esophageal SCC, and hypermethylation of the promoter region might be one of the crucial pathways to silence RUNX3 gene.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Runx2  (RUNX family transcription factor 2)
Runx3  (RUNX family transcription factor 3)

Genes (Mus musculus)
Runx2  (runt related transcription factor 2)
Runx3  (runt related transcription factor 3)

Genes (Homo sapiens)
RUNX2  (RUNX family transcription factor 2)
RUNX3  (RUNX family transcription factor 3)


Additional Information