RGD Reference Report - Adaptor protein complexes 1 and 3 are essential for generation of synaptic vesicles from activity-dependent bulk endosomes. - Rat Genome Database

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Adaptor protein complexes 1 and 3 are essential for generation of synaptic vesicles from activity-dependent bulk endosomes.

Authors: Cheung, Giselle  Cousin, Michael A 
Citation: Cheung G and Cousin MA, J Neurosci. 2012 Apr 25;32(17):6014-23. doi: 10.1523/JNEUROSCI.6305-11.2012.
RGD ID: 12050120
Pubmed: PMID:22539861   (View Abstract at PubMed)
PMCID: PMC3348540   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.6305-11.2012   (Journal Full-text)

Activity-dependent bulk endocytosis is the dominant synaptic vesicle retrieval mode during high intensity stimulation in central nerve terminals. A key event in this endocytosis mode is the generation of new vesicles from bulk endosomes, which replenish the reserve vesicle pool. We have identified an essential requirement for both adaptor protein complexes 1 and 3 in this process by employing morphological and optical tracking of bulk endosome-derived synaptic vesicles in rat primary neuronal cultures. We show that brefeldin A inhibits synaptic vesicle generation from bulk endosomes and that both brefeldin A knockdown and shRNA knockdown of either adaptor protein 1 or 3 subunits inhibit reserve pool replenishment from bulk endosomes. Conversely, no plasma membrane function was found for adaptor protein 1 or 3 in either bulk endosome formation or clathrin-mediated endocytosis. Simultaneous knockdown of both adaptor proteins 1 and 3 indicated that they generated the same population of synaptic vesicles. Thus, adaptor protein complexes 1 and 3 play an essential dual role in generation of synaptic vesicles during activity-dependent bulk endocytosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ap1g1Ratsynaptic vesicle budding from endosome involved_inIDA PMID:22539861SynGO 
Ap1g1Ratsynaptic vesicle budding from endosome involved_inIMP PMID:22539861SynGO 
Ap3d1Ratsynaptic vesicle budding from endosome involved_inIDA PMID:22539861SynGO 
Ap3d1Ratsynaptic vesicle budding from endosome involved_inIMP PMID:22539861SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Ap1g1  (adaptor related protein complex 1 subunit gamma 1)
Ap3d1  (adaptor related protein complex 3 subunit delta 1)


Additional Information