RGD Reference Report - Molecular anatomy of a trafficking organelle. - Rat Genome Database
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Molecular anatomy of a trafficking organelle.

Authors: Takamori, Shigeo  Holt, Matthew  Stenius, Katinka  Lemke, Edward A  Grønborg, Mads  Riedel, Dietmar  Urlaub, Henning  Schenck, Stephan  Brügger, Britta  Ringler, Philippe  Müller, Shirley A  Rammner, Burkhard  Gräter, Frauke  Hub, Jochen S  De Groot, Bert L  Mieskes, Gottfried  Moriyama, Yoshinori  Klingauf, Jürgen  Grubmüller, Helmut  Heuser, John  Wieland, Felix  Jahn, Reinhard 
Citation: Takamori S, etal., Cell. 2006 Nov 17;127(4):831-46.
RGD ID: 12050113
Pubmed: (View Article at PubMed) PMID:17110340
DOI: Full-text: DOI:10.1016/j.cell.2006.10.030

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire trafficking organelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.


Gene Ontology Annotations    

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Atp6v0a1  (ATPase H+ transporting V0 subunit a1)
Atp6v1b1  (ATPase H+ transporting V1 subunit B1)
Exoc4  (exocyst complex component 4)
Lamp1  (lysosomal-associated membrane protein 1)
Rab11b  (RAB11B, member RAS oncogene family)
Rab3a  (RAB3A, member RAS oncogene family)
Rab3b  (RAB3B, member RAS oncogene family)
Rab5a  (RAB5A, member RAS oncogene family)
Rab5al1  (Rab5a, member RAS oncogene family-like 1)
Rph3a  (rabphilin 3A)
Sec22b  (SEC22 homolog B, vesicle trafficking protein)
Stx12  (syntaxin 12)
Stx16  (syntaxin 16)
Stx2  (syntaxin 2)
Stx6  (syntaxin 6)
Stx7  (syntaxin 7)
Stxbp5  (syntaxin binding protein 5)
Sv2a  (synaptic vesicle glycoprotein 2a)
Syn1  (synapsin I)
Syngr1  (synaptogyrin 1)
Sypl1  (synaptophysin-like 1)
Syt1  (synaptotagmin 1)
Vamp2  (vesicle-associated membrane protein 2)
Vamp3  (vesicle-associated membrane protein 3)
Vamp7  (vesicle-associated membrane protein 7)
Vps33b  (VPS33B, late endosome and lysosome associated)
Wfs1  (wolframin ER transmembrane glycoprotein)

Additional Information