RGD Reference Report - Nanoscale scaffolding domains within the postsynaptic density concentrate synaptic AMPA receptors. - Rat Genome Database

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Nanoscale scaffolding domains within the postsynaptic density concentrate synaptic AMPA receptors.

Authors: MacGillavry, Harold D  Song, Yu  Raghavachari, Sridhar  Blanpied, Thomas A 
Citation: MacGillavry HD, etal., Neuron. 2013 May 22;78(4):615-22. doi: 10.1016/j.neuron.2013.03.009.
RGD ID: 12050098
Pubmed: PMID:23719161   (View Abstract at PubMed)
PMCID: PMC3668352   (View Article at PubMed Central)
DOI: DOI:10.1016/j.neuron.2013.03.009   (Journal Full-text)

Scaffolding molecules at the postsynaptic membrane form the foundation of excitatory synaptic transmission by establishing the architecture of the postsynaptic density (PSD), but the small size of the synapse has precluded measurement of PSD organization in live cells. We measured the internal structure of the PSD in live neurons at approximately 25 nm resolution using photoactivated localization microscopy (PALM). We found that four major PSD scaffold proteins were each organized in distinctive ~80 nm ensembles able to undergo striking changes over time. Bidirectional PALM and single-molecule immunolabeling showed that dense nanodomains of PSD-95 were preferentially enriched in AMPA receptors more than NMDA receptors. Chronic suppression of activity triggered changes in PSD interior architecture that may help amplify synaptic plasticity. The observed clustered architecture of the PSD controlled the amplitude and variance of simulated postsynaptic currents, suggesting several ways in which PSD interior organization may regulate the strength and plasticity of neurotransmission.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glutamatergic synapse is_active_inIDA 12050098PMID:23719161SynGO 
glutamatergic synapse is_active_inIMP 12050098PMID:23719161SynGO 
postsynaptic density is_active_inIDA 12050098PMID:23719161SynGO 
postsynaptic density is_active_inIMP 12050098PMID:23719161SynGO 
postsynaptic density located_inIDA 12050098PMID:23719161SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Shank3  (SH3 and multiple ankyrin repeat domains 3)


Additional Information