RGD Reference Report - Global metabolic profiling for the study of Rhizoma Paridis saponins-induced hepatotoxicity in rats. - Rat Genome Database

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Global metabolic profiling for the study of Rhizoma Paridis saponins-induced hepatotoxicity in rats.

Authors: Man, Shuli  Qiu, Peiyu  Li, Jing  Zhang, Liming  Gao, Wenyuan 
Citation: Man S, etal., Environ Toxicol. 2017 Jan;32(1):99-108. doi: 10.1002/tox.22215. Epub 2015 Nov 21.
RGD ID: 11576319
Pubmed: PMID:26590097   (View Abstract at PubMed)
DOI: DOI:10.1002/tox.22215   (Journal Full-text)

Rhizoma Paridis saponins (RPS) is a traditional Chinese medicine (TCM) from the plant Paris polyphylla var. yunnanensis (Fr.) Hand.-Mazz. Despite its potentially clinical utility such as anticancer and anti-inflammation, it has slight side effects and toxicity as previous report. In this work, 90-day administration of RPS induced liver injury. (1) H-NMR- and GC/MS-based metabonomic analyses in conjunction with histopathological examinations, blood biochemistry and hepatic phase I and II enzymes assays were performed to evaluate the toxic mechanisms of RPS induced in rats. As a result, oral administration of RPS possessed certain liver toxicity in SD rats. (1) H-NMR and GC/MS data indicated that RPS inhibited the oxidation of fatty acids, glycolysis, and TCA cycle pathway, and disturbed glycine, serine, and threonine metabolism. Low expression of TG, T-CHO, and LDL-C and high levels of ALT and AST indicated that chronic exposure to RPS caused hepatocyte damage, synthesis dysfunction, and transportation failure of lipoproteins. In addition, RPS downregulated the mRNA levels of CYP1A2, CYP2E1, and UGTs. In conclusion, we used metabonomics approach to study the toxicity of RPS for the first time. This research demonstrated that metabonomics method was a promising tool to study and diagnose TCM-induced toxicity. © 2015 Wiley Periodicals, Inc. Environ Toxicol 32: 99-108, 2017.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Liver Injury  ISOCyp1a2 (Rattus norvegicus)11576319; 11576319mRNA:decreased expression:liverRGD 
Liver Injury  IEP 11576319mRNA:decreased expression:liverRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp1a2  (cytochrome P450, family 1, subfamily a, polypeptide 2)

Genes (Mus musculus)
Cyp1a2  (cytochrome P450, family 1, subfamily a, polypeptide 2)

Genes (Homo sapiens)
CYP1A2  (cytochrome P450 family 1 subfamily A member 2)


Additional Information