RGD Reference Report - Negative regulation of the rat Na-K-ATPase alpha 3-subunit gene promoter by thyroid hormone. - Rat Genome Database
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Negative regulation of the rat Na-K-ATPase alpha 3-subunit gene promoter by thyroid hormone.

Authors: He, H  Chin, S  Zhuang, K  Hartong, R  Apriletti, J  Gick, G 
Citation: He H, etal., Am J Physiol. 1996 Nov;271(5 Pt 1):C1750-6.
RGD ID: 11576286
Pubmed: (View Article at PubMed) PMID:8944660

Na-K-ATPase alpha 3-subunit mRNA levels are both positively and negatively controlled by thyroid hormone [3,5,3'triiodothyronine (T3)] in primary cultures of neonatal rat cardiac myocytes. In this study, transient transfection analysis indicated that two regions of the rat alpha 3 gene between nucleotides -116 and -6 and -6 and +80 conferred T3-mediated inhibition of reporter gene expression. Electrophoretic mobility shift assays showed specific binding of T3 receptor monomers and T3 receptor-retinoid X receptor heterodimers at each alpha 3 gene negative T3-response region. The alpha 3 gene region from -116 to -6 base pairs also mediates repression in response to retinoic acid (RA) and binds RA receptor. In the absence of ligand, reporter gene expression driven by the -116 to -6-base pair region is repressed with cotransfection of T3 receptor, whereas it is unaffected by overexpression of RA receptor. These data demonstrate that the proximal promoter of the rat Na-K-ATPase alpha 3 gene contains sequence motifs that mediate repression of alpha 3 gene transcription in response to either T3 or RA in neonatal rat cardiac myocytes.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Atp1a3  (ATPase Na+/K+ transporting subunit alpha 3)


Additional Information