RGD Reference Report - Role of autophosphorylation in regulation of protein kinase CK2 from rat neuronal chromatin. - Rat Genome Database

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Role of autophosphorylation in regulation of protein kinase CK2 from rat neuronal chromatin.

Authors: Kulikova, OG  Reikhardt, BA 
Citation: Kulikova OG and Reikhardt BA, Biochemistry (Mosc). 1998 Dec;63(12):1400-6.
RGD ID: 11565845
Pubmed: PMID:9916157   (View Abstract at PubMed)

The regulation of rat brain cortex protein kinase CK2 (casein kinase 2) by autophosphorylation has been investigated. Purified CK2 from rat neuronal chromatin is composed of two regulatory (beta) and two catalytic (alpha and/or alpha;) subunits. The molecular masses of the subunits--43 (alpha), 39 (alpha;), and 25 kD (beta)--were similar to those of typical CK2 subunits. A significant amount of alpha;-subunit occurred in neuronal chromatin; the molar ratios for the subunits were 1.1:0.9:1.9. Pharmacological probes (derivatives of 4,5-di(N-methylcarbamoyl)-1-alkyl-imidazole) were used to study autophosphorylation of separate subunits. These compounds have different effects on neuronal chromatin CK2, transcription, and neurological memory. Changes in the autophosphorylation of the CK2 subunits were found with the help of these compounds. Inhibitors of transcription decreased the beta-subunit autophosphorylation. Stimulators of transcription increased the beta-subunit autophosphorylation and promoted the autophosphorylation of the alpha; (but not the alpha) subunit. The beta-subunit autophosphorylation led to reduction in phosphorylation of nonhistone HMG 14 protein that has been shown to be a physiological substrate of CK2. The autophosphorylation of the alpha;-subunit raised the CK2 activity with HMG 14. The question of functional distinctions between the alpha;- and alpha-subunits of chromatin CK2 in differentiated neurons is discussed.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Csnk2a1Ratchromatin  IDA  RGD 
Csnk2a2Ratchromatin  IDA  RGD 
Csnk2bRatchromatin  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csnk2a1  (casein kinase 2 alpha 1)
Csnk2a2  (casein kinase 2 alpha 2)
Csnk2b  (casein kinase 2 beta)

Additional Information