RGD Reference Report - Heterogeneous nuclear ribonucleoprotein A2 interacts with protein kinase CK2. - Rat Genome Database

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Heterogeneous nuclear ribonucleoprotein A2 interacts with protein kinase CK2.

Authors: Pancetti, F  Bosser, R  Krehan, A  Pyerin, W  Itarte, E  Bachs, O 
Citation: Pancetti F, etal., Biochem Biophys Res Commun. 1999 Jun 24;260(1):17-22.
RGD ID: 11565844
Pubmed: PMID:10381337   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1999.0849   (Journal Full-text)

The catalytic subunit of protein kinase CK2 (CK2alpha) was found associated with heterogeneous nuclear ribonucleoprotein particles (hnRNPs) that contain the core proteins A2 and C1-C2. High levels of CK2 activity were also detected in these complexes. Phosphopeptide patterns of hnRNP A2 phosphorylated in vivo and in vitro by protein kinase CK2 were similar, suggesting that this kinase can phosphorylate hnRNPA2 in vivo. Binding experiments using human recombinant hnRNP A2, free human recombinant CK2alpha or CK2beta subunits, reconstituted CK2 holoenzyme and purified native rat liver CK2 indicated that hnRNP A2 associated with both catalytic and regulatory CK2 subunits, and that the interaction was independent of the presence of RNA. However, the capability of hnRNP A2 to bind to CK2 holoenzyme was lower than its binding to the isolated subunits. These data indicate that the association of CK2alpha with CK2beta interferes with the subsequent binding of hnRNP A2. HnRNP A2 inhibited the autophosphorylation of CK2beta. This effect was stronger with reconstituted human recombinant CK2 than with purified native rat liver CK2.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Csnk2a1Ratribonucleoprotein complex binding  IDA  RGD 
Csnk2bRatribonucleoprotein complex binding  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csnk2a1  (casein kinase 2 alpha 1)
Csnk2b  (casein kinase 2 beta)

Additional Information