RGD Reference Report - Differential gene expression in infarct scar and viable myocardium from rat heart following coronary ligation. - Rat Genome Database

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Differential gene expression in infarct scar and viable myocardium from rat heart following coronary ligation.

Authors: Xu, YJ  Chapman, D  Dixon, IM  Sethi, R  Guo, X  Dhalla, NS 
Citation: Xu YJ, etal., J Cell Mol Med. 2004 Jan-Mar;8(1):85-92.
RGD ID: 11565830
Pubmed: PMID:15090263   (View Abstract at PubMed)
PMCID: PMC6740260   (View Article at PubMed Central)

Post-myocardial infarction (MI) remodeling of cardiac myocytes and the myocardial interstitium results in alteration of gross ventricular geometry and ventricular dysfunction. To investigate the mechanisms of the remodeling process of the heart after large MI, the expression of various genes in viable left ventricle and infarct scar tissue were examined at 16 weeks post-MI. Steady-state expression of Na(+)-K+ ATPase alpha-1 and -2, phospholamban (PLB), alpha-myosin heavy chain (alpha-MHC), ryanodine receptor (Rya) and Ca2+ ATPase (Serca2) mRNAs were decreased in the infarct scar vs noninfarcted sham-operated controls (P < 0.05). On the other hand, Gialpha2 and beta-MHC mRNAs were upregulated (P < 0.05, respectively) in the infarct scar whereas Na(+)-K+ ATPase-beta, Na(+)-Ca2+ exchanger and Gs mRNAs were not altered vs control values. In viable left ventricle, the alpha-1 subunit of Na(+)-K+ ATPase, alpha-3, beta-isoforms, Rya, beta-MHC, Gialpha2, Gs and Na(+)-Ca2+ exchanger were significantly elevated while expression of the alpha-2 subunit of Na(+)-K+ ATPase, PLB and Serca2 were significantly decreased compared to controls. Expression of CK2alpha mRNA was elevated in noninfarcted heart (145 +/- 15%) and diminished in the infarct scar (66 +/- 13%) vs controls. Expression of beta-MHC mRNA was elevated in both viable and infarct scar tissues of experimental hearts (140 +/- 31% and 183 +/- 30% vs. controls, respectively). These results suggest that cardiac genes in the infarcted tissue and viable left ventricle following MI are differentially regulated.

RGD Manual Disease Annotations    Click to see Annotation Detail View


Objects Annotated

Genes (Rattus norvegicus)
Csnk2a1  (casein kinase 2 alpha 1)
Csnk2b  (casein kinase 2 beta)
Myh6  (myosin heavy chain 6)
Myh7  (myosin heavy chain 7)

Genes (Mus musculus)
Csnk2a1  (casein kinase 2, alpha 1 polypeptide)
Csnk2b  (casein kinase 2, beta polypeptide)
Myh6  (myosin, heavy polypeptide 6, cardiac muscle, alpha)
Myh7  (myosin, heavy polypeptide 7, cardiac muscle, beta)

Genes (Homo sapiens)
CSNK2A1  (casein kinase 2 alpha 1)
CSNK2B  (casein kinase 2 beta)
MYH6  (myosin heavy chain 6)
MYH7  (myosin heavy chain 7)

Additional Information