RGD Reference Report - Differential localization of alpha' and beta subunits of protein kinase CK2 during rat spermatogenesis. - Rat Genome Database

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Differential localization of alpha' and beta subunits of protein kinase CK2 during rat spermatogenesis.

Authors: Alvarado-Diaz, CP  Tapia, JC  Antonelli, M  Moreno, RD 
Citation: Alvarado-Diaz CP, etal., Cell Tissue Res. 2009 Oct;338(1):139-49. doi: 10.1007/s00441-009-0847-1. Epub 2009 Aug 27.
RGD ID: 11565138
Pubmed: PMID:19711102   (View Abstract at PubMed)
DOI: DOI:10.1007/s00441-009-0847-1   (Journal Full-text)

Protein kinase CK2 is a serine/threonine kinase expressed in organisms from yeast to human and is composed of a catalytic subunit (alpha or alpha') and a regulatory subunit (beta) forming a holoenzyme with the possible subunit combinations alpha(2)beta(2), alpha'(2)beta(2), or alphaalpha'beta(2). This kinase has been shown to be involved in embryonic development and gametogenesis. We have studied the expression of the CK2alpha' and CK2beta subunits during the first wave of spermatogenesis and in adult testis in the rat. Western blot analyses have demonstrated that both CK2alpha' and CK2beta are expressed in testes from birth to adulthood. A more detailed study of the protein localization of CK2alpha' and CK2beta by immunohistochemistry suggests that CK2alpha' and CK2beta are localized in the nuclei of Sertoli cells in 5-day-old rats, whereas they appear to have a cytoplasmic localization in older animals. In adult testes, CK2alpha' and CK2beta subunits are present in spermatocytes. Both subunits exhibit a similar expression pattern with the highest level in spermatocytes at stages VIII-XIV. Interestingly, CK2beta is highly expressed in spermatogonia, whereas CK2alpha' is barely detectable. Mature epididymal spermatozoa express CK2alpha' in the acrosome and CK2beta in the flagellum. This new evidence therefore indicates that protein kinase CK2 has a possible role at various stages during mammalian spermatogenesis, a process that involves proliferation, meiosis, apoptosis, and differentiation. CK2 might thus emerge as a new pivotal control enzyme at various levels in mammalian spermatogenesis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Csnk2a2Ratspermatogenesis  IEP  RGD 

Cellular Component

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Csnk2a2Ratacrosomal vesicle  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Csnk2a2  (casein kinase 2 alpha 2)

Objects referenced in this article
Gene Csnk2a1 casein kinase 2 alpha 1 Rattus norvegicus
Gene Csnk2b casein kinase 2 beta Rattus norvegicus

Additional Information