RGD Reference Report - Effects of creatine and beta-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models. - Rat Genome Database

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Effects of creatine and beta-guanidinopropionic acid and alterations in creatine transporter and creatine kinases expression in acute seizure and chronic epilepsy models.

Authors: Kim, DW  Yeo, SI  Ryu, HJ  Kim, JE  Song, HK  Kwon, OS  Choi, SY  Kang, TC 
Citation: Kim DW, etal., BMC Neurosci. 2010 Oct 28;11:141. doi: 10.1186/1471-2202-11-141.
RGD ID: 11565113
Pubmed: PMID:20979657   (View Abstract at PubMed)
PMCID: PMC2978220   (View Article at PubMed Central)
DOI: DOI:10.1186/1471-2202-11-141   (Journal Full-text)

BACKGROUND: In order to confirm the roles of creatine (Cr) in epilepsy, we investigated the anti-convulsive effects of Cr, creatine transporter (CRT) and creatine kinases (CKs) against chemical-induced acute seizure activity and chronic epileptic seizure activity. RESULTS: Two hr after pilocarpine (PILO)-seizure induction, ubiquitous mitochondrial CK (uMtCK) immunoreactivity was unaltered as compared to control level. However, brain-type cytoplasm CK (BCK) immunoreactivity was decreased to 70% of control level. CRT immunoreactivity was decreased to 60% of control level. Following Cr or Tat-CK treatment, uMtCK or CRT immunoreactivity was unaffected, while BCK immunoreactivity in Cr treated group was increased to 3.6-fold of control levels. beta-Guanidinopropionic acid (GPA, a competitive CRT inhibitor) reduced BCK and CRT expression. In addition, Cr and tat-BCK treatment delayed the beginning of seizure activity after PILO injection. However, GPA treatment induced spontaneous seizure activity without PILO treatment. In chronic epilepsy rats, both uMtCK and CRT immunoreactivities were reduced in the hippocampus. In contrast, BCK immunoreactivity was similar to that observed in control animals. Cr-, GPA and tat-BCK treatment could not change EEG. CONCLUSION: Cr/CK circuit may play an important role in sustaining or exacerbating acute seizure activity, but not chronic epileptic discharge.

RGD Manual Disease Annotations    Click to see Annotation Detail View

Objects Annotated

Genes (Rattus norvegicus)
Ckmt1  (creatine kinase, mitochondrial 1)
Slc6a8  (solute carrier family 6 member 8)

Genes (Mus musculus)
Ckmt1  (creatine kinase, mitochondrial 1, ubiquitous)
Slc6a8  (solute carrier family 6 (neurotransmitter transporter, creatine), member 8)

Genes (Homo sapiens)
CKMT1B  (creatine kinase, mitochondrial 1B)
SLC6A8  (solute carrier family 6 member 8)


Additional Information