RGD Reference Report - Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. - Rat Genome Database

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Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice.

Authors: Wang, X  Wang, S  Li, C  Gao, T  Liu, Y  Rangiani, A  Sun, Y  Hao, J  George, A  Lu, Y  Groppe, J  Yuan, B  Feng, JQ  Qin, C 
Citation: Wang X, etal., PLoS Genet. 2012;8(5):e1002708. doi: 10.1371/journal.pgen.1002708. Epub 2012 May 17.
RGD ID: 11558021
Pubmed: PMID:22615579   (View Abstract at PubMed)
PMCID: PMC3355082   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pgen.1002708   (Journal Full-text)

Family with sequence similarity 20,-member C (FAM20C) is highly expressed in the mineralized tissues of mammals. Genetic studies showed that the loss-of-function mutations in FAM20C were associated with human lethal osteosclerotic bone dysplasia (Raine Syndrome), implying an inhibitory role of this molecule in bone formation. However, in vitro gain- and loss-of-function studies suggested that FAM20C promotes the differentiation and mineralization of mouse mesenchymal cells and odontoblasts. Recently, we generated Fam20c conditional knockout (cKO) mice in which Fam20c was globally inactivated (by crossbreeding with Sox2-Cre mice) or inactivated specifically in the mineralized tissues (by crossbreeding with 3.6 kb Col 1a1-Cre mice). Fam20c transgenic mice were also generated and crossbred with Fam20c cKO mice to introduce the transgene in the knockout background. In vitro gain- and loss-of-function were examined by adding recombinant FAM20C to MC3T3-E1 cells and by lentiviral shRNA-mediated knockdown of FAM20C in human and mouse osteogenic cell lines. Surprisingly, both the global and mineralized tissue-specific cKO mice developed hypophosphatemic rickets (but not osteosclerosis), along with a significant downregulation of osteoblast differentiation markers and a dramatic elevation of fibroblast growth factor 23 (FGF23) in the serum and bone. The mice expressing the Fam20c transgene in the wild-type background showed no abnormalities, while the expression of the Fam20c transgene fully rescued the skeletal defects in the cKO mice. Recombinant FAM20C promoted the differentiation and mineralization of MC3T3-E1 cells. Knockdown of FAM20C led to a remarkable downregulation of DMP1, along with a significant upregulation of FGF23 in both human and mouse osteogenic cell lines. These results indicate that FAM20C is a bone formation "promoter" but not an "inhibitor" in mouse osteogenesis. We conclude that FAM20C may regulate osteogenesis through its direct role in facilitating osteoblast differentiation and its systemic regulation of phosphate homeostasis via the mediation of FGF23.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Hypophosphatemic Rickets  ISOFam20c (Mus musculus)11558021; 11558021 RGD 
Hypophosphatemic Rickets  IMP 11558021 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fam20c  (FAM20C, golgi associated secretory pathway kinase)

Genes (Mus musculus)
Fam20c  (FAM20C, golgi associated secretory pathway kinase)

Genes (Homo sapiens)
FAM20C  (FAM20C golgi associated secretory pathway kinase)


Additional Information