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[Destruction of mRNA poly-A sequences by polynucleotide phosphorylase in rat liver microsomes].

Authors: Del'vig, AA 
Citation: Del'vig AA Biokhimiia. 1978 Nov;43(11):1983-8.
Pubmed: (View Article at PubMed) PMID:737213

A possible role of polynucleotide phosphorylase (PNPase) in the destruction of poly-A fragments located at the 3'-OH end of mRNA from rat liver polyribosomes was studied. Using hybridization of mRNP particles and mRNA with poly-U Sepharose 4B, it was found that polyribosomal PNPase in vitro destroys the poly-A sequences of approximately 25% of poly-A+ mRNA during the first minutes of incubation at a high rate. The destruction of the poly-A fragment of mRNA by PNPase is incomplete, since part of it is presumably protected by proteins firmly bound to the poly-A sequences of mRNA.

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RGD ID: 11554176
Created: 2016-10-20
Species: All species
Last Modified: 2016-10-20
Status: ACTIVE



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