RGD Reference Report - Cytokines in experimental otitis media with effusion. - Rat Genome Database

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Cytokines in experimental otitis media with effusion.

Authors: Johnson, MD  Fitzgerald, JE  Leonard, G  Burleson, JA  Kreutzer, DL 
Citation: Johnson MD, etal., Laryngoscope. 1994 Feb;104(2):191-6.
RGD ID: 11554174
Pubmed: PMID:8302123   (View Abstract at PubMed)
DOI: DOI:10.1288/00005537-199402000-00012   (Journal Full-text)

Studies in the authors' laboratory have recently demonstrated the presence of potent inflammatory cytokines such as interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF alpha) in human middle ear effusions. The clinical significance of this finding has not been fully elucidated because of the limitations of human studies. We hypothesized that the chinchilla model of otitis media may be an appropriate system with which to study the role of cytokines in otitis media with effusion. To begin to investigate this possibility, 30 chinchillas underwent surgical blockage of the eustachian tube (ET) to promote effusion development. After 2 weeks, examination by otoscopy demonstrated 27 ears to have developed an effusion. Next, all middle ear clefts, in random manner, were either injected with heat-killed Streptococcus pneumoniae 1 x 10(6) in 0.1 mL normal saline, injected with 0.1 mL normal saline alone, or received no injection at all. Middle ear effusions were obtained and analyzed for IL-1 beta and TNF alpha by enzyme-linked immunosorbent assay (ELISA). This study demonstrated a significant correlation between IL-1 beta and the presence of an effusion (P < .001). Additionally, increased TNF alpha levels correlated with bacterial component presence (P < .001), i.e., mean TNF alpha level was 108, 10.8, and 0 pg/mL in bacteria, normal saline, and noninjected ears, respectively. These findings would suggest that cytokine expression may relate to specific pathological conditions and that the chinchilla model for otitis media with effusion (OME) could be used to further explore the role of cytokines in OME.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IL1BHumanOtitis Media with Effusion  ISOIl1b (Chinchilla lanigera) RGD 
Il1bChinchillaOtitis Media with Effusion  IEP  RGD 
TNFHumanStreptococcal Infections  ISOTnf (Chinchilla lanigera)associated with Otitis MediaRGD 
TnfChinchillaStreptococcal Infections  IEP associated with Otitis MediaRGD 

Objects Annotated

Genes (Homo sapiens)
IL1B  (interleukin 1 beta)
TNF  (tumor necrosis factor)

Genes (Chinchilla lanigera)
Il1b  (interleukin 1 beta)
Tnf  (tumor necrosis factor)


Additional Information