RGD Reference Report - Middle ear fluid cytokine and inflammatory cell kinetics in the chinchilla otitis media model. - Rat Genome Database

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Middle ear fluid cytokine and inflammatory cell kinetics in the chinchilla otitis media model.

Authors: Sato, K  Liebeler, CL  Quartey, MK  Le, CT  Giebink, GS 
Citation: Sato K, etal., Infect Immun. 1999 Apr;67(4):1943-6.
RGD ID: 11553900
Pubmed: PMID:10085040   (View Abstract at PubMed)
PMCID: PMC96550   (View Article at PubMed Central)

Streptococcus pneumoniae is the most frequent microbe causing middle ear infection. The pathophysiology of pneumococcal otitis media has been characterized by measurement of local inflammatory mediators such as inflammatory cells, lysozyme, oxidative metabolic products, and inflammatory cytokines. The role of cytokines in bacterial infection has been elucidated with animal models, and interleukin (IL)-1beta, IL-6, and IL-8 and tumor necrosis factor alpha (TNF-alpha) are recognized as being important local mediators in acute inflammation. We characterized middle ear inflammatory responses in the chinchilla otitis media model after injecting a very small number of viable pneumococci into the middle ear, similar to the natural course of infection. Middle ear fluid (MEF) concentrations of IL-1beta, IL-6, IL-8, and TNF-alpha were measured by using anti-human cytokine enzyme-linked immunosorbent assay reagents. IL-1beta showed the earliest peak, at 6 h after inoculation, whereas IL-6, IL-8, and TNF-alpha concentrations were increasing 72 h after pneumococcal inoculation. IL-6, IL-8, and TNF-alpha but not IL-1beta concentrations correlated significantly with total inflammatory cell numbers in MEF, and all four cytokines correlated significantly with MEF neutrophil concentration. Several intercytokine correlations were significant. Cytokines, therefore, participate in the early middle ear inflammatory response to S. pneumoniae.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CXCL8Humanotitis media  ISOCxcl8 (Chinchilla lanigera) RGD 
Cxcl8Chinchillaotitis media  IEP  RGD 
IL1BHumanotitis media  ISOIl1b (Chinchilla lanigera) RGD 
IL6Humanotitis media  ISOIl6 (Chinchilla lanigera) RGD 
Il1bChinchillaotitis media  IEP  RGD 
Il6Chinchillaotitis media  IEP  RGD 
TNFHumanotitis media  ISOTnf (Chinchilla lanigera) RGD 
TnfChinchillaotitis media  IEP  RGD 

Objects Annotated

Genes (Homo sapiens)
CXCL8  (C-X-C motif chemokine ligand 8)
IL1B  (interleukin 1 beta)
IL6  (interleukin 6)
TNF  (tumor necrosis factor)

Genes (Chinchilla lanigera)
Cxcl8  (C-X-C motif chemokine ligand 8)
Il1b  (interleukin 1 beta)
Il6  (interleukin 6)
Tnf  (tumor necrosis factor)


Additional Information