RGD Reference Report - Cholesterol depletion uncouples beta-dystroglycans from discrete sarcolemmal domains, reducing the mechanical activity of skeletal muscle. - Rat Genome Database

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Cholesterol depletion uncouples beta-dystroglycans from discrete sarcolemmal domains, reducing the mechanical activity of skeletal muscle.

Authors: Vega-Moreno, J  Tirado-Cortes, A  Alvarez, R  Irles, C  Mas-Oliva, J  Ortega, A 
Citation: Vega-Moreno J, etal., Cell Physiol Biochem. 2012;29(5-6):905-18. doi: 10.1159/000186933. Epub 2012 May 11.
RGD ID: 11541063
Pubmed: PMID:22613990   (View Abstract at PubMed)
DOI: DOI:10.1159/000186933   (Journal Full-text)

BACKGROUND/AIMS: beta-Dystroglycan (beta-DG) is a transmembrane glycoprotein that links the intracellular cytoskeleton to the extracellular matrix and is crucial for the molecular pathway of lateral force transmission in muscle. We aimed to investigate the effect of decreasing sarcolemmal cholesterol on the distribution of beta-DG, its interaction with dystrophin and the impact on the contraction efficiency of muscle. METHODS: Isolated rat extensor digitorum longus muscles were incubated with methyl beta-cyclodextrin (MbetaCD) to deplete cholesterol and with MbetaCD-cholesterol to restore cholesterol. Electric stimulation protocols were used to determine muscle force and fatigue. Detergent-resistant membranes (lipid rafts) were separated from isolated skeletal muscle sarcolemma. The distribution and interactions of beta-DG, caveolin-3 and dystrophin were determined by an immunoreactivity analysis. RESULTS: Cholesterol depletion in muscle results in a weakened force of contraction, which recovers after cholesterol restoration. The rate of fatigue is unaffected, but fatigue recovery is dependent upon cholesterol restoration. MbetaCD modifies the structures of lipid rafts obtained from MbetaCD-treated muscles by, displacing the membrane proteins beta-DG and caveolin-3 f from the lipid raft, thus reducing the interaction of beta-DG with dystrophin. CONCLUSION: Cholesterol depletion weakens the muscle contractile force by disturbing the sarcolemmal distribution of beta-dystroglycan and its interaction with dystrophin, two key proteins in the alignment of lateral force transmission pathway.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to cholesterol  IEP 11541063 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane raft  IDA 11541063 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Dag1  (dystroglycan 1)


Additional Information