RGD Reference Report - Molecular cloning and characterization of rat Pomt1 and Pomt2. - Rat Genome Database

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Molecular cloning and characterization of rat Pomt1 and Pomt2.

Authors: Manya, H  Chiba, A  Margolis, RU  Endo, T 
Citation: Manya H, etal., Glycobiology. 2006 Sep;16(9):863-73. Epub 2006 May 16.
RGD ID: 11532759
Pubmed: (View Article at PubMed) PMID:16704966
DOI: Full-text: DOI:10.1093/glycob/cwl002

Mammalian O-mannosylation, although an uncommon type of protein modification, is essential for normal brain and muscle development. Defective O-mannosylation causes congenital muscular dystrophy with abnormal neuronal migration [Walker-Warburg syndrome (WWS)]. Here, we have identified and cloned rat Pomt1 and Pomt2, which are homologues of human POMT1 and POMT2, with identities of 86 and 90%, respectively, at the amino acid level. Coexpression of both genes was found to be necessary for enzymatic activity, as is the case with human POMT1 and POMT2. Northern blot and reverse transcriptase polymerase chain reaction (RT-PCR) analyses revealed that rat Pomt1 and Pomt2 are expressed in all tissues but most strongly in testis. In situ hybridization histochemistry of rat brain revealed that Pomt1 and Pomt2 mRNA are coexpressed in neurons (dentate gyrus and CA1-CA3 region of the hippocampus and cerebellar Purkinje cells). Two transcription-initiation sites were observed in rat Pomt2, resulting in two forms: a testis form and a somatic form. The two forms had equal protein O-mannosyltransferase activity when coexpressed with rat Pomt1. Coexpression studies also showed that the human and rat protein O-mannosyltransferases are interchangeable, providing further evidence for the closeness of their structures.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Pomt1  (protein-O-mannosyltransferase 1)
Pomt2  (protein-O-mannosyltransferase 2)


Additional Information