RGD Reference Report - Stimulation of c-jun and c-myb in rat Sertoli cells following exposure to retinoids. - Rat Genome Database
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Stimulation of c-jun and c-myb in rat Sertoli cells following exposure to retinoids.

Authors: Page, KC  Heitzman, DA  Chernin, MI 
Citation: Page KC, etal., Biochem Biophys Res Commun. 1996 May 15;222(2):595-600.
RGD ID: 11532681
Pubmed: (View Article at PubMed) PMID:8670250
DOI: Full-text: DOI:10.1006/bbrc.1996.0789

Circulating hormones are engaged in initiating and controlling the process of spermatogenesis, however, the Sertoli cell tight junctions prevent these substances from directly influencing the spermatogenic cells. Thus, the Sertoli cells play a vital role in regulating the spermatogenic process. Our studies correlate retinoid action with the transactivation of genes which encode the transcription factors, Jun and Myb, in the Sertoli cells of the rat testis, a retinol-dependent organ. We examined the mRNA levels for the protooncogene, c-jun, a member of the AP-I transcription complex, as well as mRNA levels for the protooncogene, c-myb, following retinoid stimulation. The major changes observed were a significant up-regulation in c-jun mRNA beginning at 30 min which reached a four-fold peak over controls at 1 h while the c-myb mRNA level exhibited a delay in up-regulation which rose abruptly to a significant three-fold peak over controls at 1 h. Interestingly, a variation in transcript size for c-jun (5.4 kb) was also detected in rat Sertoli cells. The significant 50% rise in the c-jun mRNA levels at 30 min prior to the rapid rise in the c-myb message at 1 h suggests that Jun may be involved in the transactivation of c-myb gene expression. These data support the model that retinoid modulation of these immediate early genes is the first step in a process which initiates a cascade of vitamin A-inducible gene expression in rat Sertoli cells necessary for maintaining spermatogenesis.

Annotation

Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Myb  (MYB proto-oncogene, transcription factor)


Additional Information