RGD Reference Report - Dynamic changes in the gene expression profile during rat oral carcinogenesis induced by 4-nitroquinoline 1-oxide. - Rat Genome Database

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Dynamic changes in the gene expression profile during rat oral carcinogenesis induced by 4-nitroquinoline 1-oxide.

Authors: Ge, S  Zhang, J  Du, Y  Hu, B  Zhou, Z  Lou, J 
Citation: Ge S, etal., Mol Med Rep. 2016 Mar;13(3):2561-9. doi: 10.3892/mmr.2016.4883. Epub 2016 Feb 8.
RGD ID: 11531126
Pubmed: PMID:26860129   (View Abstract at PubMed)
PMCID: PMC4768982   (View Article at PubMed Central)
DOI: DOI:10.3892/mmr.2016.4883   (Journal Full-text)

The typical progression of oral cancer is from hyperplastic epithelial lesions through dysplasia to invasive carcinoma. It is important to investigate malignant oral cancer progression and development in order to determine useful approaches of prevention of dysplastic lesions. The present study aimed to gain insights into the underlying molecular mechanism of oral carcinogenesis by establishing a rat model of oral carcinogenesis using 4nitroquinoline 1oxide. Subsequently, transcription profile analysis using an integrating microarray was performed. The dynamic gene expression changes of the six stages of rat oral carcinogenesis (normal, mild epithelial dysplasia, moderate dysplasia, severe dysplasia, carcinoma in situ and oral squamous cell carcinomas) were analyzed using component plane presentations (CPP)selforganizing map (SOM). Six genes were verified by quantitative polymerase chain reaction, immunohistochemistry and succinate dehydrogenase (SDH) activity assay kit. Numerous differentially expressed genes (DEGs) were identified during rat oral carcinogenesis. CPPSOM determined that these DEGs were primarily enriched during cell cycle, apoptosis, inflammatory response and tricarboxylic acid cycle, indicating the coordinated regulation of molecular networks. In addition, the expression of specific DEGs, such as janus kinase 3, cyclindependent kinase A1, Bcell chronic lymphocytic leukaemia/lymphoma 2like 2, nuclear factorkappaB, tumor necrosis factor receptor superfamily member 1A, cyclin D1 and SDH were identified to have high concordance with the results from microarray data. The current study demonstrated that oral carcinogenesis is a multistep and multigene process, with a distinct pattern alteration along a continuum of malignant transformation. In addition, this comprehensive investigation provided a theoretical basis for the understanding of the molecular alterations associated with oral carcinogenesis.

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Jak3  (Janus kinase 3)

Genes (Mus musculus)
Jak3  (Janus kinase 3)

Genes (Homo sapiens)
JAK3  (Janus kinase 3)

Additional Information