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Organization of the Arp2/3 complex in hippocampal spines.

Authors: Racz, B  Weinberg, RJ 
Citation: Racz B and Weinberg RJ, J Neurosci. 2008 May 28;28(22):5654-9. doi: 10.1523/JNEUROSCI.0756-08.2008.
Pubmed: (View Article at PubMed) PMID:18509026
DOI: Full-text: DOI:10.1523/JNEUROSCI.0756-08.2008

Changes in the morphology of a dendritic spine require remodeling of its actin-based cytoskeleton. Biochemical mechanisms underlying actin remodeling have been studied extensively, but little is known about the physical organization of the actin-binding proteins that mediate remodeling in spines. Long-term potentiation-inducing stimuli trigger expansion of the spine head, suggesting local extension and branching of actin filaments. Because filament branching requires the Arp2/3 complex, we used quantitative immunoelectron microscopy to elucidate the organization of ARPC-2 (Arp2/3 complex subunit 2), an essential component of the complex. Our data from CA1 hippocampus indicate that Arp2/3 concentrates within spines in a previously unrecognized torroidal domain, apparently specialized to mediate actin filament branching.


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RGD Object Information
RGD ID: 11530067
Created: 2016-08-24
Species: All species
Last Modified: 2016-08-24
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.