RGD Reference Report - Intravenous transfusion of endothelial colony-forming cells attenuates vascular degeneration after cerebral aneurysm induction. - Rat Genome Database
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Intravenous transfusion of endothelial colony-forming cells attenuates vascular degeneration after cerebral aneurysm induction.

Authors: Li, S  Tian, Y  Huang, X  Zhang, Y  Wang, D  Wei, H  Dong, J  Jiang, R  Zhang, J 
Citation: Li S, etal., Brain Res. 2014 Dec 17;1593:65-75. doi: 10.1016/j.brainres.2014.09.077. Epub 2014 Oct 12.
RGD ID: 11522723
Pubmed: (View Article at PubMed) PMID:25316629
DOI: Full-text: DOI:10.1016/j.brainres.2014.09.077

Cerebral aneurysm (CA) rupture is a major cause of subarachnoid hemorrhage with high morbidity and mortality. Using an animal model, we examined the potential of endothelial colony-forming cells (ECFCs) transfusion on vascular degeneration after CA induction and underlying mechanisms. CA was induced in the right anterior cerebral artery-olfactory artery (ACA/OA) bifurcations in Sprague-Dawley rats with or without ECFCs transfusion. The degeneration of internal elastic lamina (IEL), media thickness and CA size were evaluated. Expression of matrix metalloproteinase-2 and 9 (MMP-2 and 9), tissue inhibitor of metalloproteinase-1 (TIMP-1), macrophage chemoattractant protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1), nuclear factor kappaB (NF-kappaB), endothelial nitric oxide synthase (eNOS), B-cell leukemia/lymphoma-2 (Bcl-2), and inducible nitric oxide synthase (iNOS) were analyzed by quantitative real-time polymerase chain reaction. The macrophages infiltration and apoptosis of smooth muscle cells (SMCs) were examined immunohistologically. Rats in CA+ECFCs transfusion group showed a notable reduction in IEL degeneration, media thinning and CA size compared with those in CA+saline group. ECFCs transfusion inhibited the MMP-driven wall destruction by downregulating MMP-2, MMP-9 expression and upregulating TIMP-1. ECFCs transfusion dramatically decreased VCAM-1 and NF-kappaB expression, increased eNOS expression and caused no change in MCP-1 expression, which was accompanied by reduced macrophages infiltration. Moreover, ECFCs transfusion reversed downregulation of Bcl-2 expression and upregulation of iNOS expression, and decreased SMCs apoptosis. Collectively, these findings suggest that ECFCs transfusion confers protection against degeneration of aneurysmal wall by inhibiting inflammatory cascades and SMCs apoptosis.

Annotation

Disease Annotations    
intracranial aneurysm  (IDA,IEP,ISO)

Objects Annotated

Genes (Rattus norvegicus)
Bcl2  (BCL2, apoptosis regulator)
Ccl2  (C-C motif chemokine ligand 2)

Genes (Mus musculus)
Bcl2  (B cell leukemia/lymphoma 2)
Ccl2  (chemokine (C-C motif) ligand 2)

Genes (Homo sapiens)
BCL2  (BCL2 apoptosis regulator)
CCL2  (C-C motif chemokine ligand 2)


Additional Information