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Induction of hexokinase I expression in normal and diabetic rats by a brassinosteroid isoform.

Authors: Muthuraman, P  Srikumar, K 
Citation: Muthuraman P and Srikumar K, Eur J Pharm Sci. 2010 Sep 11;41(1):1-9. doi: 10.1016/j.ejps.2010.05.008. Epub 2010 May 16.
Pubmed: (View Article at PubMed) PMID:20570616
DOI: Full-text: DOI:10.1016/j.ejps.2010.05.008

This study aimed to investigate the effect of the plant growth regulator 28-homobrassinolide (HB) on the hexokinase I (HK I) enzyme gene expression in the tissues of normal and streptozotocin-induced diabetic rats. Normal and diabetic rats were administered 50 microg of HB for 15 consecutive days. The tissues level of HK I mRNA expression was quantitated by PCR and densitometry analysis, HK I protein expression was quantitated by Western blot and densitometry analysis, localization of HK I was done by immunohistochemistry and HK enzyme activity was determined by coupled enzyme assay. Subchronic treatment of rats with HB enhanced HK I enzyme expression in diabetic rat compared to the control rat. From these experimental evidences, we came to the conclusion that HK I played a vital role in the regulation of blood sugar in streptozotocin-induced diabetic rats. A direct role for hexokinase enzyme activity in the control of diabetes is presented.


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RGD Object Information
RGD ID: 11353964
Created: 2016-07-27
Species: All species
Last Modified: 2016-07-27
Status: ACTIVE


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