RGD Reference Report - Leiomodin is an actin filament nucleator in muscle cells. - Rat Genome Database

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Leiomodin is an actin filament nucleator in muscle cells.

Authors: Chereau, D  Boczkowska, M  Skwarek-Maruszewska, A  Fujiwara, I  Hayes, DB  Rebowski, G  Lappalainen, P  Pollard, TD  Dominguez, R 
Citation: Chereau D, etal., Science. 2008 Apr 11;320(5873):239-43. doi: 10.1126/science.1155313.
RGD ID: 11344939
Pubmed: PMID:18403713   (View Abstract at PubMed)
PMCID: PMC2845909   (View Article at PubMed Central)
DOI: DOI:10.1126/science.1155313   (Journal Full-text)

Initiation of actin polymerization in cells requires nucleation factors. Here we describe an actin-binding protein, leiomodin, that acted as a strong filament nucleator in muscle cells. Leiomodin shared two actin-binding sites with the filament pointed end-capping protein tropomodulin: a flexible N-terminal region and a leucine-rich repeat domain. Leiomodin also contained a C-terminal extension of 150 residues. The smallest fragment with strong nucleation activity included the leucine-rich repeat and C-terminal extension. The N-terminal region enhanced the nucleation activity threefold and recruited tropomyosin, which weakly stimulated nucleation and mediated localization of leiomodin to the middle of muscle sarcomeres. Knocking down leiomodin severely compromised sarcomere assembly in cultured muscle cells, which suggests a role for leiomodin in the nucleation of tropomyosin-decorated filaments in muscles.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lmod2Ratsarcomere organization involved_inIMP PMID:18403713UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Lmod2RatM band located_inIDA PMID:18403713UniProt 
Lmod2Ratsarcomere located_inIDA PMID:18403713UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Lmod2  (leiomodin 2)


Additional Information