RGD Reference Report - Dissociation and dispersion of claudin-3 from the tight junction could be one of the most sensitive indicators of reflux esophagitis in a rat model of the disease. - Rat Genome Database

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Dissociation and dispersion of claudin-3 from the tight junction could be one of the most sensitive indicators of reflux esophagitis in a rat model of the disease.

Authors: Oguro, M  Koike, M  Ueno, T  Asaoka, D  Mori, H  Nagahara, A  Uchiyama, Y  Watanabe, S 
Citation: Oguro M, etal., J Gastroenterol. 2011 May;46(5):629-38. doi: 10.1007/s00535-011-0390-1. Epub 2011 Mar 5.
RGD ID: 11344880
Pubmed: PMID:21373849   (View Abstract at PubMed)
DOI: DOI:10.1007/s00535-011-0390-1   (Journal Full-text)

BACKGROUND: The aim of this study was to characterize pathological lesions of the esophageal epithelial tight junction (TJ) complex in a rat reflux esophagitis (RE) model in a search for a reliable diagnostic indicator. METHODS: Rats underwent an operation to induce RE, with or without rabeprazole treatment (1.0 and 10.0 mg/kg/day). Sham-operated rats served as a control. Fourteen days after the operation, esophagi were isolated from the rats and submitted to double-label confocal immunofluorescence microscopy, and biochemical analyses. RESULTS: Immunofluorescence microscopy revealed that claudins-1, -3, and -4 were located on the surfaces of epithelial cells in the normal esophagus of the control group, although there were differences in the distribution patterns between claudin-3 and claudins-1 and -4 in the epithelial layer. However, in RE, the immunoreactivity of claudin-3 on the cell surface was decreased, and it appeared instead as a faint granular pattern within the epithelial cytoplasm. Claudin-3 expression in the entire esophageal epithelium was also decreased. The expression and location of claudins-1 and -4 in epithelial cells were basically unaffected in RE. Gastric acid-induced dissociation of claudin-3 elicited instability of the epithelial TJ complex, which was confirmed by sedimentation analysis using centrifugation in a sucrose density gradient. Rabeprazole (10.0 mg/kg/day) attenuated these alterations. CONCLUSIONS: Our data indicate that the dispersion of claudin-3 from esophageal epithelial plasma membranes to cytoplasm and the resulting instability of the TJ complex could be one of the most specific and sensitive indicators for monitoring inflammatory and recovery processes in RE.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CLDN3Humanpeptic esophagitis  ISOCldn3 (Rattus norvegicus)protein:altered expression:epithelial cell and esophagus:RGD 
Cldn3Ratpeptic esophagitis  IEP protein:altered expression:epithelial cell and esophagus:RGD 
Cldn3Mousepeptic esophagitis  ISOCldn3 (Rattus norvegicus)protein:altered expression:epithelial cell and esophagus:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cldn3  (claudin 3)

Genes (Mus musculus)
Cldn3  (claudin 3)

Genes (Homo sapiens)
CLDN3  (claudin 3)


Additional Information