RGD Reference Report - Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. - Rat Genome Database

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Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis.

Authors: Wang, C  Wang, Y  Hu, M  Chai, Z  Wu, Q  Huang, R  Han, W  Zhang, CX  Zhou, Z 
Citation: Wang C, etal., EMBO Rep. 2016 Jan;17(1):47-63. doi: 10.15252/embr.201540689. Epub 2015 Nov 20.
RGD ID: 11079192
Pubmed: PMID:26589353   (View Abstract at PubMed)
PMCID: PMC4718405   (View Article at PubMed Central)
DOI: DOI:10.15252/embr.201540689   (Journal Full-text)

Precise and efficient endocytosis is essential for vesicle recycling during a sustained neurotransmission. The regulation of endocytosis has been extensively studied, but inhibitors have rarely been found. Here, we show that synaptotagmin-11 (Syt11), a non-Ca(2+)-binding Syt implicated in schizophrenia and Parkinson's disease, inhibits clathrin-mediated endocytosis (CME) and bulk endocytosis in dorsal root ganglion neurons. The frequency of both types of endocytic event increases in Syt11 knockdown neurons, while the sizes of endocytosed vesicles and the kinetics of individual bulk endocytotic events remain unaffected. Specifically, clathrin-coated pits and bulk endocytosis-like structures increase on the plasma membrane in Syt11-knockdown neurons. Structural-functional analysis reveals distinct domain requirements for Syt11 function in CME and bulk endocytosis. Importantly, Syt11 also inhibits endocytosis in hippocampal neurons, implying a general role of Syt11 in neurons. Taken together, we propose that Syt11 functions to ensure precision in vesicle retrieval, mainly by limiting the sites of membrane invagination at the early stage of endocytosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syt11Ratnegative regulation of clathrin coat assembly involved_inIMP PMID:26589353ParkinsonsUK-UCL 
Syt11Ratnegative regulation of clathrin-dependent endocytosis involved_inIMP PMID:26589353ParkinsonsUK-UCL 
Syt11Ratnegative regulation of membrane invagination involved_inIMP PMID:26589353ParkinsonsUK-UCL 
Syt11Ratnegative regulation of synaptic vesicle endocytosis involved_inIMP PMID:26589353ParkinsonsUK-UCL 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Syt11Rataxon located_inIDA PMID:26589353ParkinsonsUK-UCL 
Syt11Ratcell body located_inIDA PMID:26589353ParkinsonsUK-UCL 
Syt11Ratvesicle located_inIDA PMID:26589353ParkinsonsUK-UCL 

Objects Annotated

Genes (Rattus norvegicus)
Syt11  (synaptotagmin 11)


Additional Information