RGD Reference Report - Transmembrane Protein 184A Is a Receptor Required for Vascular Smooth Muscle Cell Responses to Heparin. - Rat Genome Database

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Transmembrane Protein 184A Is a Receptor Required for Vascular Smooth Muscle Cell Responses to Heparin.

Authors: Pugh, RJ  Slee, JB  Farwell, SL  Li, Y  Barthol, T  Patton, WA  Lowe-Krentz, LJ 
Citation: Pugh RJ, etal., J Biol Chem. 2016 Mar 4;291(10):5326-41. doi: 10.1074/jbc.M115.681122. Epub 2016 Jan 14.
RGD ID: 11059583
Pubmed: PMID:26769966   (View Abstract at PubMed)
PMCID: PMC4777864   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M115.681122   (Journal Full-text)

Vascular cell responses to exogenous heparin have been documented to include decreased vascular smooth muscle cell proliferation following decreased ERK pathway signaling. However, the molecular mechanism(s) by which heparin interacts with cells to induce those responses has remained unclear. Previously characterized monoclonal antibodies that block heparin binding to vascular cells have been found to mimic heparin effects. In this study, those antibodies were employed to isolate a heparin binding protein. MALDI mass spectrometry data provide evidence that the protein isolated is transmembrane protein 184A (TMEM184A). Commercial antibodies against three separate regions of the TMEM184A human protein were used to identify the TMEM184A protein in vascular smooth muscle cells and endothelial cells. A GFP-TMEM184A construct was employed to determine colocalization with heparin after endocytosis. Knockdown of TMEM184A eliminated the physiological responses to heparin, including effects on ERK pathway activity and BrdU incorporation. Isolated GFP-TMEM184A binds heparin, and overexpression results in additional heparin uptake. Together, these data support the identification of TMEM184A as a heparin receptor in vascular cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane located_inIDA 11059583PMID:26769966UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Tmem184a  (transmembrane protein 184A)


Additional Information