RGD Reference Report - VAMP4 Is an Essential Cargo Molecule for Activity-Dependent Bulk Endocytosis. - Rat Genome Database
Submit Data |  Help |  Video Tutorials |  News |  Publications |  FTP Download |  REST API |  Citing RGD |  Contact   

VAMP4 Is an Essential Cargo Molecule for Activity-Dependent Bulk Endocytosis.

Authors: Nicholson-Fish, JC  Kokotos, AC  Gillingwater, TH  Smillie, KJ  Cousin, MA 
Citation: Nicholson-Fish JC, etal., Neuron. 2015 Dec 2;88(5):973-84. doi: 10.1016/j.neuron.2015.10.043. Epub 2015 Nov 19.
RGD ID: 11058152
Pubmed: (View Article at PubMed) PMID:26607000
DOI: Full-text: DOI:10.1016/j.neuron.2015.10.043

The accurate formation of synaptic vesicles (SVs) and incorporation of their protein cargo during endocytosis is critical for the maintenance of neurotransmission. During intense neuronal activity, a transient and acute accumulation of SV cargo occurs at the plasma membrane. Activity-dependent bulk endocytosis (ADBE) is the dominant SV endocytosis mode under these conditions; however, it is currently unknown how ADBE mediates cargo retrieval. We examined the retrieval of different SV cargo molecules during intense stimulation using a series of genetically encoded pH-sensitive reporters in neuronal cultures. The retrieval of only one reporter, VAMP4-pHluorin, was perturbed by inhibiting ADBE. This selective recovery was confirmed by the enrichment of endogenous VAMP4 in purified bulk endosomes formed by ADBE. VAMP4 was also essential for ADBE, with a cytoplasmic di-leucine motif being critical for this role. Therefore, VAMP4 is the first identified ADBE cargo and is essential for this endocytosis mode to proceed.


Gene Ontology Annotations    

Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Vamp4  (vesicle-associated membrane protein 4)

Additional Information