RGD Reference Report - Recruitment of the Arp2/3 complex and mena for the stimulation of actin polymerization in growth cones by nerve growth factor. - Rat Genome Database

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Recruitment of the Arp2/3 complex and mena for the stimulation of actin polymerization in growth cones by nerve growth factor.

Authors: Goldberg, D J  Foley, M S  Tang, D  Grabham, P W 
Citation: Goldberg DJ, etal., J Neurosci Res. 2000 May 15;60(4):458-67.
RGD ID: 11049163
Pubmed: PMID:10797548   (View Abstract at PubMed)
DOI: DOI:10.1002/(SICI)1097-4547(20000515)60:4<458::AID-JNR4>3.0.CO;2-Z   (Journal Full-text)

The growth of axons and dendrites during development and regeneration is regulated by cues in the environment. Many of these cues regulate the actin cytoskeleton of the protrusive structures (like filopodia) of the growth cone that are essential for detecting and responding to cues. Nerve growth factor, which promotes the formation of protrusive structures, stimulated actin polymerization in rat sympathetic growth cones, resulting within 1-2 min in accumulations of F-actin at the distal edge and in splotches of F-actin farther back. We examined the potential involvement of a protein machinery important in at least certain types of actin polymerization in non-neuronal cells. Members of the Arp2/3 complex, p34-Arc and p21-Arc, heavily concentrated in the early accumulations of F-actin, as did one member of the Ena/VASP family (Mena) but not another (VASP). Retention of Arc proteins at preferred sites of actin polymerization did not require polymerization itself. Growth cones of differentiated PC12 cells were similar to sympathetic growth cones in their response to NGF. Introduction into these cells of a peptide that should block the binding of Ena/VASP family proteins to the protein complex at sites of actin polymerization reduced the formation of splotches and filopodia in response to NGF. These results point to the early involvement of the Arp2/3 complex and the Ena/VASP family in growth factor-stimulated actin polymerization that gives rise to protrusive structures at the growth cone.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Arpc2Ratcellular response to nerve growth factor stimulus  IEP  RGD 
Arpc3Ratcellular response to nerve growth factor stimulus  IEP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Arpc2Ratfilamentous actin  IDA  RGD 
Arpc3Ratfilamentous actin  IDA  RGD 
Arpc2Ratgrowth cone  IDA  RGD 
Arpc3Ratgrowth cone leading edge  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Arpc2  (actin related protein 2/3 complex, subunit 2)
Arpc3  (actin related protein 2/3 complex, subunit 3)


Additional Information