RGD Reference Report - Altered mucosal DNA methylation in parallel with highly active Helicobacter pylori-related gastritis. - Rat Genome Database

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Altered mucosal DNA methylation in parallel with highly active Helicobacter pylori-related gastritis.

Authors: Yoshida, T  Kato, J  Maekita, T  Yamashita, S  Enomoto, S  Ando, T  Niwa, T  Deguchi, H  Ueda, K  Inoue, I  Iguchi, M  Tamai, H  Ushijima, T  Ichinose, M 
Citation: Yoshida T, etal., Gastric Cancer. 2013 Oct;16(4):488-97. doi: 10.1007/s10120-012-0230-x. Epub 2013 Jan 6.
RGD ID: 11046273
Pubmed: PMID:23292007   (View Abstract at PubMed)
DOI: DOI:10.1007/s10120-012-0230-x   (Journal Full-text)

BACKGROUND: Chronic inflammation triggered by Helicobacter pylori causes altered DNA methylation in stomach mucosae, which is deeply involved in gastric carcinogenesis. This study aimed to elucidate the correlation between altered mucosal DNA methylation levels and activity of H. pylori-related gastritis, because inflammatory activity shows particular correlations with the development of diffuse-type cancer. METHODS: Methylation levels in stomach mucosae of 78 healthy volunteers were determined by real-time methylation-specific PCR or bisulfite pyrosequencing. Examined loci were the promoter CpG islands of six genes (FLNc, HAND1, THBD, p41ARC, HRASLS, and LOX) and the CpG sites of non-coding repetitive elements (Alu and Satalpha) that are reportedly altered by H. pylori infection. Activity of H. pylori-related gastritis was evaluated using two serum markers: H. pylori antibody titer and pepsinogen II. RESULTS: Methylation levels of the six CpG islands were consistently increased, and those of the two repetitive elements were consistently decreased in a stepwise manner with the activity of gastric inflammation as represented by serum marker levels. Each serum marker level was well correlated with the overall DNA methylation status of stomach mucosa, and these two serologic markers were additive in the detection of the mucosa with severely altered DNA methylation. CONCLUSIONS: Alteration in mucosal DNA methylation level was closely correlated with activity of H. pylori-related gastritis as evaluated by serum markers. The observed correlation between altered DNA methylation levels and activity of H. pylori-related gastritis appears to be one of the relevant molecular mechanisms underlying the development of diffuse-type cancer.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
gastritis  IAGP 11046273DNA:hypermethylation:promoter: (human)RGD 
gastritis  ISOARPC1B (Homo sapiens)11046273; 11046273DNA:hypermethylation:promoter: (human)RGD 

Phenotype Annotations    Click to see Annotation Detail View

Manual Human Phenotype Annotations - RGD

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Abnormal circulating protein concentration  IAGP 11046273DNA:hypermethylation:promoter:RGD 
Helicobacter pylori infection  IAGP 11046273DNA:hypermethylation:promoter:RGD 
Objects Annotated

Genes (Rattus norvegicus)
Arpc1b  (actin related protein 2/3 complex, subunit 1B)

Genes (Mus musculus)
Arpc1b  (actin related protein 2/3 complex, subunit 1B)

Genes (Homo sapiens)
ARPC1B  (actin related protein 2/3 complex subunit 1B)


Additional Information