RGD Reference Report - Borneol Depresses P-Glycoprotein Function by a NF-kappaB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model. - Rat Genome Database
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Borneol Depresses P-Glycoprotein Function by a NF-kappaB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model.

Authors: Fan, X  Chai, L  Zhang, H  Wang, Y  Zhang, B  Gao, X 
Citation: Fan X, etal., Int J Mol Sci. 2015 Nov 18;16(11):27576-88. doi: 10.3390/ijms161126051.
RGD ID: 11040997
Pubmed: (View Article at PubMed) PMID:26593909
DOI: Full-text: DOI:10.3390/ijms161126051

P-glycoprotein (P-gp) on brain microvascular endothelial cells (BMECs) that form the blood brain barrier (BBB), influences transportation of substances between blood and brain. The objective of this study was to characterize the effects of borneol on P-gp efflux function on BBB and explore the potential mechanisms. We established an in vitro BBB model comprised of rat BMECs and astrocytes to measure the effects of borneol on the known P-gp substrates transport across BBB, and examined the function and expression of P-gp in BMECs and the signaling pathways regulating P-gp expression. Borneol increased intracellular accumulation of Rhodamine 123, enhanced verapamil and digoxin across the BBB in vitro model, and depressed mdr1a mRNA and P-gp expression. Borneol could activate nuclear factor-kappaB (NF-kappaB) and inhibition of NF-kappaB with MG132 (carbobenzoxy-Leu-Leu-leucinal) and SN50 (an inhibitory peptide) obscuring the P-gp decreases induced by borneol. These data suggested that borneol depresses P-gp function in BMECs by a NF-kappaB signaling medicated mechanism in a BBB in vitro model.

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Biological Process

Objects Annotated

Genes (Rattus norvegicus)
Abcb1a  (ATP binding cassette subfamily B member 1A)


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