RGD Reference Report - A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice. - Rat Genome Database

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A novel human CD32 mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic mice.

Authors: Van Royen-Kerkhof, A  Sanders, EA  Walraven, V  Voorhorst-Ogink, M  Saeland, E  Teeling, JL  Gerritsen, A  Van Dijk, MA  Kuis, W  Rijkers, GT  Vitale, L  Keler, T  McKenzie, SE  Leusen, JH  Van De Winkel, JG 
Citation: van Royen-Kerkhof A, etal., Br J Haematol. 2005 Jul;130(1):130-7.
RGD ID: 11040945
Pubmed: PMID:15982355   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1365-2141.2005.05571.x   (Journal Full-text)

A fully human IgG1 kappa antibody (MDE-8) was generated, which recognised Fc-gamma receptor IIa (FcgammaRIIa) molecules on CD32 transfectants, peripheral blood monocytes, polymorphonuclear cells and platelets. This antibody blocked FcgammaRIIa ligand-binding via its F(ab')(2) fragment. Overnight incubation of monocytes with F(ab')(2) fragments of MDE-8 leads to a c. 60% decrease in cell surface expression of FcgammaRIIa. MDE-8 whole antibody induced a concomitant c. 30% decrease of FcgammaRI on THP-1 cells and monocytes. In humans FcgammaRIIa plays an important role in the clearance of antibody-coated red blood cells in vivo. As an equivalent of FcgammaRIIa does not exist in mice, the in vivo effect of MDE-8 was studied in an FcgammaRIIa transgenic mouse model. In these mice, antibody-induced anaemia could readily be blocked by MDE-8. These data document a new human antibody that effectively blocks FcgammaRIIa, induces modulation of both FcgammaRIIa and FcgammaRI from phagocytic cells, and ameliorates antibody-induced anaemia in vivo.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FCGR2AHumanhemolytic anemia  IMP  RGD 
Fcgr2aRathemolytic anemia  ISOFCGR2A (Homo sapiens) RGD 
Fcgr3Mousehemolytic anemia  ISOFCGR2A (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fcgr2a  (Fc gamma receptor 2A)

Genes (Mus musculus)
Fcgr3  (Fc receptor, IgG, low affinity III)

Genes (Homo sapiens)
FCGR2A  (Fc gamma receptor IIa)


Additional Information