RGD Reference Report - Induction of granulocyte colony-stimulating factor mRNA by focal cerebral ischemia and cortical spreading depression. - Rat Genome Database
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Induction of granulocyte colony-stimulating factor mRNA by focal cerebral ischemia and cortical spreading depression.

Authors: Kleinschnitz, C  Schroeter, M  Jander, S  Stoll, G 
Citation: Kleinschnitz C, etal., Brain Res Mol Brain Res. 2004 Nov 24;131(1-2):73-8.
RGD ID: 11039425
Pubmed: (View Article at PubMed) PMID:15530654
DOI: Full-text: DOI:10.1016/j.molbrainres.2004.08.011

Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor with neuroprotective and antiinflammatory properties. By real-time polymerase chain reaction we show that G-CSF transcripts are induced 485-fold at 4 h and 65-fold at 16 h in ischemic lesions after middle cerebral artery occlusion compared to control brains. Further analysis in photochemically induced focal ischemia revealed that G-CSF induction involved both the infarct area and remote nonischemic brain regions. Remote responses could be blocked by the noncompetitive NMDA receptor antagonist MK-801, suggesting periinfarct depolarizations as a trigger. To further confirm this notion, cortical spreading depression (CSD) was induced by focal application of KCl to the brain surface. CSD led to a 90-fold increase in G-CSF mRNA. Contrastingly, the induction of granulocyte-monocyte (GM)-CSF, another member of the hematopoietic growth factor family, was only moderate (sixfold) and restricted to ischemic brain lesions. In conclusion, G-CSF induction in the brain may be part of an intrinsic stress response aimed at limitation of neuronal damage.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Csf3  (colony stimulating factor 3)

Genes (Mus musculus)
Csf3  (colony stimulating factor 3 (granulocyte))

Genes (Homo sapiens)
CSF3  (colony stimulating factor 3)


Additional Information