RGD Reference Report - Granulocyte colony-stimulating factor protects the brain against experimental stroke via inhibition of apoptosis and inflammation. - Rat Genome Database
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Granulocyte colony-stimulating factor protects the brain against experimental stroke via inhibition of apoptosis and inflammation.

Authors: Solaroglu, I  Cahill, J  Tsubokawa, T  Beskonakli, E  Zhang, JH 
Citation: Solaroglu I, etal., Neurol Res. 2009 Mar;31(2):167-72. doi: 10.1179/174313209X393582.
RGD ID: 11039420
Pubmed: (View Article at PubMed) PMID:19298757
DOI: Full-text: DOI:10.1179/174313209X393582

OBJECTIVES: The molecular mechanisms of the anti-apoptotic and anti-inflammatory properties of granulocyte-colony stimulating factor (G-CSF) following focal cerebral ischemia in rats were examined in this study. METHODS: Sprague-Dawley rats were randomly divided into three groups: sham, middle cerebral artery occlusion (MCAO) non-treatment and MCAO with G-CSF treatment. Focal ischemia was induced with the suture occlusion method for 90 minutes, and treatment was given at the onset of reperfusion. All animals were killed 24 hours after reperfusion. Assessment included neurological scores, infarction volumes, histology, immunofluorescent staining and Western blotting. RESULTS: G-CSF significantly reduced the infarct volume and ameliorated the early neurological outcome scores. Western blot analysis showed that G-CSF treatment significantly elevated the cIAP2 levels and decreased the activation of caspase 3 in the ischemic cortex compared with the non-treated rats. Immunofluorescent works also showed that G-CSF treatment inhibited both neuronal and glial tumor necrosis factor alpha and interleukin 1beta expressions. DISCUSSION: The neuronal anti-apoptotic action of G-CSF may be mediated in part by the anti-apoptotic protein cIAP2. G-CSF also exerts anti-inflammatory actions after focal cerebral ischemia by preventing both neuronal and glial pro-inflammatory cytokine expressions.

Annotation

Disease Annotations    

Objects Annotated

Genes (Rattus norvegicus)
Csf3  (colony stimulating factor 3)

Genes (Mus musculus)
Csf3  (colony stimulating factor 3 (granulocyte))

Genes (Homo sapiens)
CSF3  (colony stimulating factor 3)


Additional Information