RGD Reference Report - Altered expression of heterogenous nuclear ribonucleoproteins and SR factors in human colon adenocarcinomas. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Altered expression of heterogenous nuclear ribonucleoproteins and SR factors in human colon adenocarcinomas.

Authors: Ghigna, C  Moroni, M  Porta, C  Riva, S  Biamonti, G 
Citation: Ghigna C, etal., Cancer Res. 1998 Dec 15;58(24):5818-24.
RGD ID: 11039405
Pubmed: PMID:9865741   (View Abstract at PubMed)

Alternative splicing is part of the expression program of a wide number of genes implicated in cell growth and differentiation. Although the occurrence of inappropriate alternative splicing in tumors has started to emerge, the underlying molecular mechanisms have been, thus far, largely unexplored. We have investigated the alternative splicing pattern of the CD44 gene in specimens of nonfamilial colon adenocarcinomas at different stages of tumor progression. In the same patients, we have assessed by Northern blotting analysis the mRNA levels of different heterogeneous nuclear ribonucleoproteins and SR factors, all involved in pre-mRNA splicing and, more in general, in mRNA maturation. The results of this analysis highlight a general rule for the mode of splicing of the CD44 pre-mRNA. Moreover, we found that the mRNA levels of different SR proteins in tumor specimens are different from, and usually lower than, those detected in samples of nonpathological tissue adjacent to the tumor. Quantitative analysis demonstrates that, in tumors, the mRNA levels of ASF, SRp40, SRp55, and SRp75, when normalized to those of heterogeneous nuclear ribonucleoprotein A1, are lower than those of SRp20 and SRp30. Interestingly, this reduction is more drastic in patients showing a more altered CD44 splicing pattern and seems to be related to the propensity to develop metastases.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SRSF4Humancolon adenocarcinoma  IEP mRNA:decreased expression:colon mucosa (human)RGD 
SRSF5Humancolon adenocarcinoma  IEP mRNA:decreased expression:colon mucosa (human)RGD 
SRSF6Humancolon adenocarcinoma  IEP mRNA:decreased expression:colon mucosa (human)RGD 
Srsf4Ratcolon adenocarcinoma  ISOSRSF4 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 
Srsf4Mousecolon adenocarcinoma  ISOSRSF4 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 
Srsf5Ratcolon adenocarcinoma  ISOSRSF5 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 
Srsf5Mousecolon adenocarcinoma  ISOSRSF5 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 
Srsf6Ratcolon adenocarcinoma  ISOSRSF6 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 
Srsf6Mousecolon adenocarcinoma  ISOSRSF6 (Homo sapiens)mRNA:decreased expression:colon mucosa (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Srsf4  (serine and arginine rich splicing factor 4)
Srsf5  (serine and arginine rich splicing factor 5)
Srsf6  (serine and arginine rich splicing factor 6)

Genes (Mus musculus)
Srsf4  (serine and arginine-rich splicing factor 4)
Srsf5  (serine and arginine-rich splicing factor 5)
Srsf6  (serine and arginine-rich splicing factor 6)

Genes (Homo sapiens)
SRSF4  (serine and arginine rich splicing factor 4)
SRSF5  (serine and arginine rich splicing factor 5)
SRSF6  (serine and arginine rich splicing factor 6)


Additional Information