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Reduced expression of MIR409-3p in primary immune thrombocytopenia.

Authors: Li, H  Zhao, H  Xue, F  Zhang, X  Zhang, D  Ge, J  Yang, Y  Xuan, M  Fu, R  Yang, R 
Citation: Li H, etal., Br J Haematol. 2013 Apr;161(1):128-35. doi: 10.1111/bjh.12213. Epub 2013 Jan 30.
Pubmed: (View Article at PubMed) PMID:23360331
DOI: Full-text: DOI:10.1111/bjh.12213

Primary immune thrombocytopenia (ITP) is an acquired autoimmune disease with many immune dysfunctions. MicroRNAs (miRNAs) are a class of non-coding RNAs that post-transcriptionally regulate gene expression by messenger RNA degradation or translational repression. Accumulating evidence has demonstrated that miRNAs play a vital role in the regulation of immunological functions and prevention of autoimmunity. However, whether miRNAs are involved in the pathogenesis of ITP is still unknown. To illustrate the role of miRNAs in ITP, the expression profile of miRNAs from peripheral blood mononuclear cells (PBMCs) in ITP patients was investigated by miRNA microarray, and further validated by TaqMan real-time polymerase chain reaction. MIR409-3p expression was decreased in PBMCs of active ITP patients, but this recovered after effective therapy. IFNG was identified and validated as one of the targeted genes of MIR409-3p by bioinformatic prediction and reporter gene analysis. In addition, we found DGCR8 transcript was down-regulated in ITP patients and positively correlated with MIR409-3p. Thus, in ITP patients, decreased DGCR8 leads to down-regulation of MIR409-3p, which in turn results in up-regulation of IFNG (IFN-gamma).


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RGD Object Information
RGD ID: 10755694
Created: 2016-02-03
Species: All species
Last Modified: 2016-02-03
Status: ACTIVE


RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.