RGD Reference Report - DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells. - Rat Genome Database

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DDX6 localizes to nuage structures and the annulus of mammalian spermatogenic cells.

Authors: Kawahara, C  Yokota, S  Fujita, H 
Citation: Kawahara C, etal., Histochem Cell Biol. 2014 Jan;141(1):111-21. doi: 10.1007/s00418-013-1153-2. Epub 2013 Oct 20.
RGD ID: 10755411
Pubmed: PMID:24141902   (View Abstract at PubMed)
DOI: DOI:10.1007/s00418-013-1153-2   (Journal Full-text)

The localization of DEAD (Asp-Glu-Ala-Asp) box helicase 6 (DDX6) in spermatogenic cells from the mouse, rat, and guinea pig was studied by immunofluorescence (IF) and immunoelectron microscopy (IEM). Spermatogenic cells from these species yielded similar DDX6 localization pattern. IF microscopy results showed that DDX6 localizes to both the nucleus and cytoplasm. In the cytoplasm of spermatogenic cells, diffuse cytosolic and discrete granular staining was observed, with the staining pattern changing during cell differentiation. IEM revealed that DDX6 localized to the five different types of nuage structures and non-nuage structures, including small granule aggregate and late spermatid annuli. Nuclear labeling was strongest in leptotene and zygotene spermatocytes and moderately strong in the nuclear pocket of late spermatids. DDX6 also localized to the surface of outer dense fibers, which comprise of flagella. The results show that DDX6 is present in nuage and non-nuage structures as well as nuclei, suggesting that DDX6 has diverse functions in spermatogenic cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
spermatid differentiation  IEP 10755411 RGD 
spermatogenesis  IEP 10755411; 10755411 RGD 

Cellular Component

Objects Annotated

Genes (Rattus norvegicus)
Ddx4  (DEAD-box helicase 4)
Ddx6  (DEAD-box helicase 6)


Additional Information