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Infliximab attenuates inflammatory osteolysis in a model of periodontitis in Wistar rats.

Authors: Goncalves, DC  Evangelista, RC  Da Silva, RR  Santos, MJ  Silva FS, JR  Aragao, KS  Brito, GA  Lucena, HB  Leitao, RC  Oria, RB 
Citation: Goncalves DC, etal., Exp Biol Med (Maywood). 2014 Apr;239(4):442-53. doi: 10.1177/1535370213520114. Epub 2014 Feb 28.
Pubmed: (View Article at PubMed) PMID:24586097
DOI: Full-text: DOI:10.1177/1535370213520114

Periodontitis is a chronic inflammatory disease related to tooth loss in adults. Infliximab is a chimeric monoclonal antibody against TNF-alpha and is prescribed for the treatment of systemic inflammatory diseases. This study aimed to investigate the role of infliximab on experimental periodontal disease (EPD). EPD was induced by passing a 3.0 nylon thread around the upper left second molar in Wistar rats. Animals were either treated with intravenous infliximab (1, 5, 7, and 10 mg/kg) or saline solution 30 min before the periodontitis induction and were followed until they were sacrificed on the 11th day. A subset of rats was euthanized on the third day for analysis of gingival myeloperoxidase (MPO) and the blood MPO granulocyte index. In addition, we analyzed the bone loss index (BLI), the periodontal histopathological score, and the periodontal collagen network using confocal microscopy. We also analyzed metalloproteinase-1/-8, RANK, RANK-L, and osteoprotegerin in maxillary tissue by immunohistochemistry Gingival MPO, IL-1beta, TNF-alpha were measured by ELISA. EPD caused leukocytosis, significant increases in BLI and gingival pro-inflammatory cytokines and cell infiltrates, with worse histopathological scores and periodontal collagen derangement. Infliximab (5 mg/kg) reduced granulocyte blood counts, gingival IL-1beta, TNF-alpha, and MPO levels, diminished MMP-1/-8, RANK, and RANK-L bone immunolabeling with better periodontal histopathological scores and collagen network in comparison with the challenged saline group. We concluded that infliximab had significant anti-inflammatory and bone-protective effects in Wistar rats challenged by periodontitis.

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RGD ID: 10450598
Created: 2016-01-19
Species: All species
Last Modified: 2016-01-19
Status: ACTIVE



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RGD is funded by grant HL64541 from the National Heart, Lung, and Blood Institute on behalf of the NIH.