RGD Reference Report - RNA interference targeting the platelet-derived growth factor receptor beta subunit ameliorates experimental hepatic fibrosis in rats. - Rat Genome Database

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RNA interference targeting the platelet-derived growth factor receptor beta subunit ameliorates experimental hepatic fibrosis in rats.

Authors: Chen, SW  Zhang, XR  Wang, CZ  Chen, WZ  Xie, WF  Chen, YX 
Citation: Chen SW, etal., Liver Int. 2008 Dec;28(10):1446-57. doi: 10.1111/j.1478-3231.2008.01759.x. Epub 2008 May 3.
RGD ID: 10449503
Pubmed: PMID:18466260   (View Abstract at PubMed)
PMCID: PMC2710794   (View Article at PubMed Central)
DOI: DOI:10.1111/j.1478-3231.2008.01759.x   (Journal Full-text)

BACKGROUND/AIMS: Platelet-derived growth factor (PDGF) is the strongest stimulator of the proliferation of hepatic stellate cells (HSCs). PDGF receptor beta subunit (PDGFR-beta) is acquired on HSCs proliferation induced by PDGF. In this study, we aim to investigate the effect of PDGFR-beta small interference RNA (siRNA) on experimental hepatic fibrosis. METHODS: We constructed a PDGFR-beta siRNA expression plasmid and investigated its effect on the activation of HSCs. Bromodeoxyuridine incorporation was performed to investigate the effect of PDGFR-beta siRNA on HSCs proliferation. A hydrodynamics-based transfection method was used to deliver PDGFR-beta siRNA to rats with hepatic fibrosis. The distribution of transgenes in the liver was observed by immunofluorescence. The antifibrogenic effect of PDGFR-beta siRNA was investigated pathologically. RESULTS: Platelet-derived growth factor receptor-beta subunit siRNA could significantly downregulate PDGFR-beta expression, suppress HSCs activation, block the mitogen-activated protein kinase signalling pathway and inhibit HSCs proliferation in vitro. PDGFR-beta siRNA expression plasmid could be delivered into activated HSCs by the hydrodynamics-based transfection method, and remarkably improve the liver function of the rat model induced by dimethylnitrosamine and bile duct ligation. Furthermore, the progression of fibrosis in the liver was significantly suppressed by PDGFR-beta siRNA in both animal models. CONCLUSIONS: Platelet-derived growth factor receptor-beta subunit siRNA may be presented as an effective antifibrogenic gene therapeutic method for hepatic fibrosis.

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Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of fibroblast proliferation  IMP 10449503 RGD 
positive regulation of hepatic stellate cell activation  IMP 10449503 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfrb  (platelet derived growth factor receptor beta)

Genes (Mus musculus)
Pdgfrb  (platelet derived growth factor receptor, beta polypeptide)

Genes (Homo sapiens)
PDGFRB  (platelet derived growth factor receptor beta)


Additional Information