RGD Reference Report - Time course of cytokine mRNA expression in kidneys of rats with unilateral ureteral obstruction. - Rat Genome Database

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Time course of cytokine mRNA expression in kidneys of rats with unilateral ureteral obstruction.

Authors: Sommer, M  Eismann, U  Deuther-Conrad, W  Wendt, T  Mohorn, T  Funfstuck, R  Stein, G 
Citation: Sommer M, etal., Nephron. 2000 Jan;84(1):49-57.
RGD ID: 10449497
Pubmed: PMID:10644908   (View Abstract at PubMed)
DOI: DOI:45538   (Journal Full-text)

The development of renal interstitial fibrosis (RIF) is related to the expression and excretion of cytokines and growth factors. Thus, we investigated the time course of mRNA expression of cytokines known as causative factors in a model of RIF in rats before and on day 10 after unilateral ureteral obstruction (UUO), when first signs of fibrosis were visible, as well as during progressive RIF. UUO causes a fivefold increase in mRNA expression of monocyte chemoattractant protein 1 15 days after surgery as compared with contralateral kidneys. The level remains elevated about three-fold up to day 25. The mRNA of the fibrogenic cytokine transforming growth factor beta 1 (TGF-beta1) is increased two- to threefold during the time course, whereas the mRNAs of platelet-derived growth factor B chain (PDGF-B) and its receptor beta (PDGF-Rbeta) increase after UUO, reaching their maxima on days 10-15. PDGF-B mRNA increase up to day 15, marking the onset of fibrosis, and decreases thereafter, whereas the expression of the PDGF-Rbeta mRNA remains elevated more than threefold over the entire study period. Incubation of cultured renal fibroblasts with TGF-beta1 and/or PDGF-B suggests that their specific action on cell growth and proliferation is maintained even when they are used in combination. The sustained elevation of TGF-beta1 and PDGF-B/PDGF-Rbeta mRNA levels confirms the assumption of a particular involvement of these cytokines in the pathogenesis of RIF. The mRNA expression of the gap junctional protein connexin 43 in ureteral ligated kidneys is increased sixfold already 5 days after UUO. In this way, the increased connexin 43 mRNA levels indicate a possible function in the remodeling of the kidney tissue after tubular damage and fibrosis.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ureteral obstruction  ISOPdgfb (Rattus norvegicus)10449497; 10449497mRNA:increased expression:kidney:RGD 
ureteral obstruction  IEP 10449497mRNA:increased expression:kidney:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pdgfb  (platelet derived growth factor subunit B)

Genes (Mus musculus)
Pdgfb  (platelet derived growth factor, B polypeptide)

Genes (Homo sapiens)
PDGFB  (platelet derived growth factor subunit B)

Additional Information