RGD Reference Report - B and T cell immune response to small nuclear ribonucleoprotein particles in lupus mice: autoreactive CD4(+) T cells recognize a T cell epitope located within the RNP80 motif of the 70K protein.

General

B and T cell immune response to small nuclear ribonucleoprotein particles in lupus mice: autoreactive CD4(+) T cells recognize a T cell epitope located within the RNP80 motif of the 70K protein.
Authors:	Monneaux, F Briand, JP Muller, S
Citation:	Monneaux F, etal., Eur J Immunol. 2000 Aug;30(8):2191-200.
RGD ID:	10448935
Pubmed:	PMID:10940910 (View Abstract at PubMed)
DOI:	DOI:10.1002/1521-4141(2000)30:8<2191::AID-IMMU2191>3.0.CO;2-R (Journal Full-text)
Systemic lupus erythematosus is characterized by the presence of high titers of autoantibodies reacting with various components of the U1 small nuclear ribonucleoprotein particle (snRNP). It has been suggested that these antibodies are produced by an antigen-driven mechanism under the dependence of antigen-specific T cells. To investigate the role of T cell help in this process, we sought, with 20 overlapping peptides, the Th epitopes on the U1-70K snRNP in unprimed H-2(k) MRL / lpr lupus mice and immunized CBA normal mice. The peptide 131 - 151 was recognized by both IgG autoantibodies and CD4(+) T cells from 7 - 9-week-old MRL / lpr mice. In this test, antigen-presenting cells (APC) from MRL / lpr mice were required; APC from naive CBA mice failed to stimulate CD4(+) cells from MRL / lpr mice. The potential role of MRL / lpr B cells as APC, the expression of MHC class II molecules at their surface and their activation state (expression of CD69, CD80 / B7-1 and CD86 / B7-2 molecules) were studied. Peptide 131 - 151 bound both I-A(k) and I-E(k) class II molecules and favored an IL-2-positive T cell response but not IFN-gamma, IL-6 and IL-10 secretion. Segment 131 - 151 is localized within the RNP80 motif and contains residues that are highly conserved in many nuclear, nucleolar and cytoplasmic RNA binding proteins.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
systemic lupus erythematosus		ISO	Snrnp70 (Mus musculus)	10448935; 10448935		RGD
systemic lupus erythematosus		IDA		10448935		RGD

Objects Annotated

Genes (Rattus norvegicus)

Snrnp70 (small nuclear ribonucleoprotein U1 subunit 70)

Genes (Mus musculus)

Snrnp70 (small nuclear ribonucleoprotein 70 (U1))

Genes (Homo sapiens)

SNRNP70 (small nuclear ribonucleoprotein U1 subunit 70)

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B and T cell immune response to small nuclear ribonucleoprotein particles in lupus mice: autoreactive CD4(+) T cells recognize a T cell epitope located within the RNP80 motif of the 70K protein.