RGD Reference Report - Neuropeptide Y protects rat cortical neurons against beta-amyloid toxicity and re-establishes synthesis and release of nerve growth factor. - Rat Genome Database

RGD Reference Report - Neuropeptide Y protects rat cortical neurons against beta-amyloid toxicity and re-establishes synthesis and release of nerve growth factor. - Rat Genome Database

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Neuropeptide Y protects rat cortical neurons against beta-amyloid toxicity and re-establishes synthesis and release of nerve growth factor.
Authors:	Croce, N Ciotti, MT Gelfo, F Cortelli, S Federici, G Caltagirone, C Bernardini, S Angelucci, F
Citation:	Croce N, etal., ACS Chem Neurosci. 2012 Apr 18;3(4):312-8. doi: 10.1021/cn200127e. Epub 2012 Jan 30.
RGD ID:	10448933
Pubmed:	PMID:22860197 (View Abstract at PubMed)
PMCID:	PMC3369802 (View Article at PubMed Central)
DOI:	DOI:10.1021/cn200127e (Journal Full-text)
Neuropeptide Y (NPY) is a 36 amino acid peptide, widely distributed within central nervous system neurons. More recently, it has been shown that NPY is involved in Alzheimer's disease (AD), a disorder characterized by accumulation of amyloid beta-peptide (Abeta) in neurons. In a previous study, we investigated the effect of NPY on neuronal damage by exposing SH-SY5Y cells (an established human derived neuroblastoma cell line) to Abeta's pathogenic fragment 25-35 (Abeta(25-35)). We found a NPY-neuroprotective action associated with changes in intracellular production of nerve growth factor (NGF), a member of the neurotrophin family. Since our results were encouraging, we decided to replicate our data using primary cortical neurons cultured in presence of Abeta(25-35), and investigated whether NPY had similar neuroprotective action. Moreover, since cortical neurons are able to produce and release NGF, we investigated whether the synthesis and release of NGF were modified in such experimental conditions. Our results showed that a preincubation with NPY counteracted the toxic effect of Abeta, as measured by increased cell viability. Moreover, NPY pretreatment had an effect on NGF since its intracellular synthesis was increased, release was normalized, and mRNA expression was downregulated. Notably, these effects on NGF were in the opposite direction of those produced by incubating the cells with Abeta alone. This study in primary cortical neurons supports the hypothesis that NPY may be a neuroprotective agent against beta-amyloid neurotoxicity. These data also suggest that NPY may influence the synthesis and the release of NGF by cortical neurons.

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Object Symbol	Species	Term	Qualifier	Evidence	With	Notes	Source	Original Reference(s)
Npy	Rat	negative regulation of cellular process		IDA		primary cortical neuron death	RGD
Npy	Rat	regulation of nerve growth factor production		IDA			RGD

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Genes (Rattus norvegicus)

Npy (neuropeptide Y)

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