RGD Reference Report - The U2 small nuclear ribonucleoprotein particle as an autoantigen. Analysis with sera from patients with overlap syndromes. - Rat Genome Database

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The U2 small nuclear ribonucleoprotein particle as an autoantigen. Analysis with sera from patients with overlap syndromes.

Authors: Craft, J  Mimori, T  Olsen, TL  Hardin, JA 
Citation: Craft J, etal., J Clin Invest. 1988 Jun;81(6):1716-24.
RGD ID: 10448928
Pubmed: PMID:2968364   (View Abstract at PubMed)
PMCID: PMC442616   (View Article at PubMed Central)
DOI: DOI:10.1172/JCI113511   (Journal Full-text)

We identified eight patients whose sera contained autoantibodies to the U2 small nuclear ribonucleoprotein (snRNP), an RNA protein particle involved in the splicing of newly transcribed messenger RNA. Each of these patients had an overlap syndrome that included features of either systemic lupus erythematosus (SLE), scleroderma, and/or polymyositis. We then used these sera to characterize the autoantigenic polypeptides of the U1 and U2 snRNP particles. In immunoblots, all sera contained antibodies to the B" polypeptide of the U2 snRNP. A subset of these sera that more effectively immunoprecipitated the native U2 particle contained an additional antibody system that recognized the A' polypeptide of this snRNP. Antibodies eluted from the B" protein bound the A polypeptide of the U1 snRNP and vice versa. Moreover, antibodies to the B" polypeptide were accompanied by antibodies to the 68K and C polypeptides of the U1 snRNP. Finally, the A' and B" polypeptides remained physically associated after the U2 particle was cleaved with RNase. Thus these sera contain multiple autoantibody systems that, at one level, target two physically associated antigenic polypeptides of the U2 particle and, at another, target two snRNP particles which are associated during the splicing of premessenger RNA. These linked autoantibody sets provide further evidence that intact macromolecular structures are targeted by the immune response in SLE and related diseases.




Objects Annotated

Genes (Rattus norvegicus)
Snrpa  (small nuclear ribonucleoprotein polypeptide A)
Snrpa1  (small nuclear ribonucleoprotein polypeptide A')
Snrpb  (small nuclear ribonucleoprotein polypeptides B and B1)
Snrpb2  (small nuclear ribonucleoprotein polypeptide B2)
Snrpc  (small nuclear ribonucleoprotein polypeptide C)

Genes (Mus musculus)
Snrpa  (small nuclear ribonucleoprotein polypeptide A)
Snrpa1  (small nuclear ribonucleoprotein polypeptide A')
Snrpb  (small nuclear ribonucleoprotein B)
Snrpb2  (U2 small nuclear ribonucleoprotein B)
Snrpc  (U1 small nuclear ribonucleoprotein C)

Genes (Homo sapiens)
SNRPA  (small nuclear ribonucleoprotein polypeptide A)
SNRPA1  (small nuclear ribonucleoprotein polypeptide A')
SNRPB  (small nuclear ribonucleoprotein polypeptides B and B1)
SNRPB2  (small nuclear ribonucleoprotein polypeptide B2)
SNRPC  (small nuclear ribonucleoprotein polypeptide C)


Additional Information