RGD Reference Report - Metallothionein-III deficiency exacerbates light-induced retinal degeneration. - Rat Genome Database

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Metallothionein-III deficiency exacerbates light-induced retinal degeneration.

Authors: Tsuruma, K  Shimazaki, H  Ohno, Y  Inoue, Y  Honda, A  Imai, S  Lee, J  Shimazawa, M  Satoh, M  Hara, H 
Citation: Tsuruma K, etal., Invest Ophthalmol Vis Sci. 2012 Nov 29;53(12):7896-903. doi: 10.1167/iovs.12-10165.
RGD ID: 10412646
Pubmed: PMID:23132798   (View Abstract at PubMed)
DOI: DOI:10.1167/iovs.12-10165   (Journal Full-text)

PURPOSE: Retinal photoreceptor damage is a common feature of ophthalmic disorders, such as age-related macular degeneration and retinitis pigmentosa. Oxidative stress has a key role in these diseases. Metallothioneins (MTs) are a family of cysteine-rich proteins, and various physiologic functions have been reported, including protection against metal toxicity and antioxidative potency. We investigated the functional role of MT-III in light-induced retinal damage. METHODS: The expression of retinal MT-I, -II, and -III mRNA was evaluated by real-time reverse-transcription PCR in retina exposed to light. Retinal damage in MT-deficient mice was induced by exposure to white light at 16,000 lux for 3 hours after dark adaptation. Photoreceptor damage was evaluated histologically by measuring the thickness of the outer nuclear layer (ONL) 5 days after light exposure and by electroretinogram recording. In an in vitro experiment, the MT-III siRNAs were tested for their effects on light-induced mouse photoreceptor cell (661W) damage. RESULTS: The mRNAs of the MTs were increased significantly in murine retina after light exposure. The ONL in the MT-III-deficient mice was remarkably thinner compared to light-exposed wild-type (WT) mice, and a- and b-wave amplitudes were decreased; the damage induced in MT-I/-II-deficient mice was comparable to that observed in WT mice. MT-III knockdown by siRNA in 661W exacerbated the cell damage and increased the production of reactive oxygen species in response to light exposure. CONCLUSIONS: These findings suggested that MT-III can help protect against light-induced retinal damage compared to MT-I/II. Some of these effects may be exerted by its antioxidative potency.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MT2AHumanretinal degeneration  ISOMt2 (Mus musculus)mRNA:increased expression:retinaRGD 
MT3Humanretinal degeneration  ISOMt3 (Mus musculus)mRNA:increased expression:retinaRGD 
Mt1Ratretinal degeneration  ISOMt1 (Mus musculus)mRNA:increased expression:retinaRGD 
Mt1Mouseretinal degeneration  IEP mRNA:increased expression:retinaRGD 
Mt2Mouseretinal degeneration  IEP mRNA:increased expression:retinaRGD 
Mt2ARatretinal degeneration  ISOMt2 (Mus musculus)mRNA:increased expression:retinaRGD 
Mt3Ratretinal degeneration  ISOMt3 (Mus musculus)mRNA:increased expression:retinaRGD 
Mt3Mouseretinal degeneration  IEP mRNA:increased expression:retinaRGD 
Mt3Mouseretinal degeneration  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mt1  (metallothionein 1)
Mt2A  (metallothionein 2A)
Mt3  (metallothionein 3)

Genes (Mus musculus)
Mt1  (metallothionein 1)
Mt2  (metallothionein 2)
Mt3  (metallothionein 3)

Genes (Homo sapiens)
MT2A  (metallothionein 2A)
MT3  (metallothionein 3)


Additional Information