RGD Reference Report - Amlodipine besylate and amlodipine camsylate prevent cortical neuronal cell death induced by oxidative stress. - Rat Genome Database

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Amlodipine besylate and amlodipine camsylate prevent cortical neuronal cell death induced by oxidative stress.

Authors: Lee, YJ  Park, HH  Koh, SH  Choi, NY  Lee, KY 
Citation: Lee YJ, etal., J Neurochem. 2011 Dec;119(6):1262-70. doi: 10.1111/j.1471-4159.2011.07529.x. Epub 2011 Nov 3.
RGD ID: 10402816
Pubmed: PMID:21988238   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1471-4159.2011.07529.x   (Journal Full-text)

We examined the neuroprotective effects of the long-acting third-generation dihydropyridine Ca(2+) antagonists, amlodipine besylate (AB) and amlodipine camsylate (AC), on neuronal cell death induced by oxidative stress. Cell viability and levels of free radicals and intracellular signaling proteins were measured after treating primary cultures of cortical neurons with AB, AC, and/or hydrogen peroxide (H(2) O(2) ) under various conditions. Cell viability was not affected by concentrations of AB or AC up to 5 muM but decreased at higher concentrations. Following H(2) O(2) exposure, the viability of cortical neurons decreased in a concentration-dependent manner; however, treatment with AB or AC up to 5 muM restored the viability of H(2) O(2) -injured cortical neurons. Treatment with H(2) O(2) increased the level of free radicals in cortical neurons, and pre-treatment with AB or AC counteracted this in a dose-dependent manner. Similarly, treatment with AB or AC reduced the declines in p85aPI3K, phosphorylated Akt, phosphorylated GSK-3beta, heat-shock transcription factor-1, and Bcl-2 induced by H(2) O(2) , as well as the increases in cyclooxygenase-2, cytosolic cytochrome c, cleaved caspase 9, and cleaved caspase 3. Our results indicate that AB and AC exert similar neuroprotective effects by reducing oxidative stress, enhancing survival signals, and inhibiting death signals.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to xenobiotic stimulus  IEP 10402816amlodipine besylate and amlodipine camsylateRGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsf1  (heat shock transcription factor 1)


Additional Information