RGD Reference Report - Proteomic analysis of synovial fibroblast-like synoviocytes from rheumatoid arthritis. - Rat Genome Database

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Proteomic analysis of synovial fibroblast-like synoviocytes from rheumatoid arthritis.

Authors: Li, XJ  Xu, M  Zhao, XQ  Zhao, JN  Chen, FF  Yu, W  Gao, DY  Luo, B 
Citation: Li XJ, etal., Clin Exp Rheumatol. 2013 Jul-Aug;31(4):552-8. Epub 2013 Jun 5.
RGD ID: 10402561
Pubmed: PMID:23739258   (View Abstract at PubMed)

OBJECTIVES: The purpose of this study was to identity protein expression patterns of fibroblast-like synoviocytes (FLSs) derived from the synovial tissue of patients with rheumatoid arthritis (RA) and osteoarthritis. METHODS: Two-dimensional gel electrophoresis (2-DE) in combination with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) was used to visualise and identify differential cellular protein expression profiles in FLSs between RA and control groups. Western-blot analysis was performed to further verify selected differentially-expressed proteins. RESULTS: A total of 1633 and 1603 protein spots were examined in synovial FLSs of RA patients and controls, respectively. Ninety-two spots in the RA group were statistically over- or under-expressed compared with controls. Among them, 33 proteins over-expressed by more than 3-fold were then identified by MALDI-TOF-MS analysis. These proteins included enzymatic and structural proteins (e.g. PKM1/M2, alpha-enolase, ERp60, lamin-A/C), signal transduction proteins (e.g. annexin 11, peroxiredoxin 1, TrpRS), heat-shock/chaperone proteins (e.g. TCP-1, GRP75, HspB5, Bip) and some unknown protein species. Three proteins, namely alpha-enolase, GRP75 and PKM2, were verified by Western blot and the results were found to be consistent with proteomic analysis. CONCLUSIONS: The differentially expressed proteins identified in RA synovial FLSs might be candidate RA-associated proteins and may prove to be promising diagnostic indicators or new therapeutic targets for RA.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
rheumatoid arthritis  IEP 10402561protein:increased expression:synovial cell:RGD 
rheumatoid arthritis  ISOHSPA9 (Homo sapiens)10402561; 10402561protein:increased expression:synovial cell:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hspa9  (heat shock protein family A (Hsp70) member 9)

Genes (Mus musculus)
Hspa9  (heat shock protein 9)

Genes (Homo sapiens)
HSPA9  (heat shock protein family A (Hsp70) member 9)


Additional Information