RGD Reference Report - Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress. - Rat Genome Database

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Aging impairs the expression of the catalytic subunit of glutamate cysteine ligase in soleus muscle under stress.

Authors: Chen, CN  Brown-Borg, HM  Rakoczy, SG  Ferrington, DA  Thompson, LV 
Citation: Chen CN, etal., J Gerontol A Biol Sci Med Sci. 2010 Feb;65(2):129-37. doi: 10.1093/gerona/glp194. Epub 2009 Dec 16.
RGD ID: 10402378
Pubmed: PMID:20018823   (View Abstract at PubMed)
PMCID: PMC2806238   (View Article at PubMed Central)
DOI: DOI:10.1093/gerona/glp194   (Journal Full-text)

This study investigated the mechanisms responsible for the disrupted homeostasis of reduced glutathione (GSH) in aging muscles with stress (14 days of hind-limb unloading [HU]). Adult and old rats were randomized into four groups: weight bearing and 3, 7, and 14 days of HU. Soleus muscles were harvested to investigate the activity or content of enzymes involved in GSH metabolism (utilization and synthesis). The activities of glutathione S transferase, glutathione reductase, gamma-glutamyl transpeptidase, and glutamate cysteine ligase (GCL) were determined. The protein content of the two subunits of GCL, catalytic subunit (GCLC) and modifier subunit (GCLM), were evaluated. The major results, failure to maintain the accelerated GCLC production and GCL activity, are associated with the GSH depletion in aging muscles with 14 days of HU. The results suggest that the regulation of GCL, especially the catalytic subunit, with stress may be compromised in aging muscles.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to activity  IEP 10402378; 10402378 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gclc  (glutamate-cysteine ligase, catalytic subunit)
Gclm  (glutamate cysteine ligase, modifier subunit)


Additional Information