RGD Reference Report - Localization of translational components at the ultramicroscopic level at postsynaptic sites of the rat brain. - Rat Genome Database

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Localization of translational components at the ultramicroscopic level at postsynaptic sites of the rat brain.

Authors: Asaki, C  Usuda, N  Nakazawa, A  Kametani, K  Suzuki, T 
Citation: Asaki C, etal., Brain Res. 2003 May 16;972(1-2):168-76.
RGD ID: 10401640
Pubmed: PMID:12711090   (View Abstract at PubMed)

We investigated the localization of components of translational machinery and their regulators in the postsynaptic region. We examined several components, especially those involved in translational regulation: components of (1) MAPK-Mnk-eIF4E, (2) PI3-kinase-PDK-Akt/PKB-FRAP/mTOR-PHAS/4EBP, (3) p70S6K-S6 ribosomal protein and (4) eEF2 kinase/CaMKIII-eEF2 pathways. Western blotting detected all the components examined in the synaptic fractions, and their differential localization to the synaptic subcompartments: initiation or elongation factors, except for eIF5, were detected predominantly in the dendritic lipid raft fraction, which contained ER marker proteins. In contrast, most of their regulatory kinases were distributed to both the postsynaptic density (PSD) and the dendritic lipid raft fractions, or enriched in the former fraction. Localization of eIF4E at synaptic sites was further examined immunohistochemically at the electron microscopic level. The eIF-4E-immunoreactivity was localized to the postsynaptic sites, especially to the microvesicle-like structures underneath the postsynaptic membrane in the spine, some of which were localized in close proximity to PSD. These results suggest that the postsynaptic local translational system, in at least four major regulatory pathways, is similar to those in the perinuclear one, and that it takes place, at least partly, immediately beneath the postsynaptic membrane. The results also suggest the presence of ER-associated type of translational machinery at the postsynaptic sites.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Eef2kRatdendritic spine located_inIDA PMID:12711090SynGO-UCL 
Eef2kRatglutamatergic synapse is_active_inIDA PMID:12711090SynGO 
Eef2kRatglutamatergic synapse is_active_inIEP PMID:12711090SynGO 
Eif4eRatglutamatergic synapse is_active_inIDA PMID:12711090SynGO 
Eif4eRatpostsynapse is_active_inIDA PMID:12711090SynGO 
Eef2kRatpostsynaptic density is_active_inIDA PMID:12711090SynGO 
Eef2kRatpostsynaptic density is_active_inIEP PMID:12711090SynGO 
Rps6kb1Ratsynapse is_active_inIDA PMID:12711090SynGO 
Rps6kb1Ratsynapse is_active_inIEP PMID:12711090SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Eef2k  (eukaryotic elongation factor-2 kinase)
Eif4e  (eukaryotic translation initiation factor 4E)
Rps6kb1  (ribosomal protein S6 kinase B1)

Objects referenced in this article
Gene Eef2 eukaryotic translation elongation factor 2 Rattus norvegicus

Additional Information