RGD Reference Report - The stoichiometry of the nucleoporin 62 subcomplex of the nuclear pore in solution. - Rat Genome Database

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The stoichiometry of the nucleoporin 62 subcomplex of the nuclear pore in solution.

Authors: Ulrich, A  Partridge, JR  Schwartz, TU 
Citation: Ulrich A, etal., Mol Biol Cell. 2014 May;25(9):1484-92. doi: 10.1091/mbc.E13-12-0745. Epub 2014 Feb 26.
RGD ID: 10401148
Pubmed: PMID:24574455   (View Abstract at PubMed)
PMCID: PMC4004597   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E13-12-0745   (Journal Full-text)

The nuclear pore complex (NPC) regulates transport between the nucleus and cytoplasm. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex contains the FG-repeat proteins Nup62, Nup54, and Nup58 and is located in the center of the NPC. The three proteins bind each other via conserved coiled-coil segments. To determine the stoichiometry of the Nup62 complex, we undertook an in vitro study using gel filtration and analytical ultracentrifugation. Our results reveal a 1:1:1 stoichiometry of the Nup62 complex, where Nup54 is central with direct binding to Nup62 and Nup58. At high protein concentration, the complex forms larger assemblies while maintaining the Nup62:Nup54:Nup58 ratio. For the homologous Nsp1 complex from Saccharomyces cerevisiae, we determine the same stoichiometry, indicating evolutionary conservation. Furthermore, we observe that eliminating one binding partner can result in the formation of complexes with noncanonical stoichiometry, presumably because unpaired coiled-coil elements tend to find a promiscuous binding partner. We suggest that these noncanonical stoichiometries observed in vitro are unlikely to be physiologically relevant.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein-containing complex  IDA 10401148 RGD 

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Nup54  (nucleoporin 54)
Nup58  (nucleoporin 58)
Nup62  (nucleoporin 62)


Additional Information