RGD Reference Report - Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways.

General

Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways.
Authors:	Shang, FF Zhao, W Zhao, Q Liu, J Li, DW Zhang, H Zhou, XF Li, CY Wang, TH
Citation:	Shang FF, etal., J Proteomics. 2013 Oct 8;91:188-99. doi: 10.1016/j.jprot.2012.12.002. Epub 2012 Dec 11.
RGD ID:	10395360
Pubmed:	PMID:23238062 (View Abstract at PubMed)
DOI:	DOI:10.1016/j.jprot.2012.12.002 (Journal Full-text)
It is well known that trauma is frequently accompanied by spontaneous functional recovery after spinal cord injury (SCI), but the underlying mechanisms remain elusive. In this study, BBB scores showed a gradual return of locomotor functions after SCT. Proteomics analysis revealed 16 differential protein spots in the gastrocnemius muscle between SCT and normal rats. Of these differential proteins, eukaryotic translation initiation factor 5A1 (elf-5A1), a highly conserved molecule throughout eukaryotes, exhibited marked upregulation in the gastrocnemius muscle after SCT. To study the role of eIF-5A1 in the restoration of hindlimb locomotor functions following SCT, we used siRNA to downregulate the mRNA level of eIF-5A1. Compared with untreated SCT control rats, those subjected to eIF-5A1 knockdown exhibited impaired functional recovery. Moreover, gene expression microarrays and bioinformatic analysis showed high correlation between three main signal pathways (ErbB, MAPK and neurotrophin signal pathways) and eIF-5A1. These signal pathways regulate cell proliferation, differentiation and neurocyte growth. Consequently, eIF-5A1 played a pivotal role via these signal pathways in hindlimb locomotor functional recovery after SCT, which could pave the way for the development of a new strategy for the treatment of spinal cord injury in clinical trials.

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Term	Qualifier	Evidence	With	Reference	Notes	Source	Original Reference(s)
Spinal Cord Injuries		ISO	Eif5a (Rattus norvegicus)	10395360; 10395360	mRNA and protein:increased expression:gastrocnemius	RGD
Spinal Cord Injuries		IEP		10395360	mRNA and protein:increased expression:gastrocnemius	RGD

Objects Annotated

Genes (Rattus norvegicus)

Eif5a (eukaryotic translation initiation factor 5A)

Genes (Mus musculus)

Eif5a (eukaryotic translation initiation factor 5A)

Genes (Homo sapiens)

EIF5A (eukaryotic translation initiation factor 5A)

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Upregulation of eIF-5A1 in the paralyzed muscle after spinal cord transection associates with spontaneous hindlimb locomotor recovery in rats by upregulation of the ErbB, MAPK and neurotrophin signal pathways.